ABSTRACT
The essential proteins NS1 and NS2 of minute virus of mice are encoded by mRNAs R1 and R2, respectively. R2 is derived from R1 by excision of a large intron and thus splicing governs the relative ratios of NS1 and NS2. Excision of the large intron utilizes a nonconsensus 5′ donor site. We identified a U-rich and A-rich intronic sequence immediately downstream of the nonconsensus 5′ donor site that functions as an intronic splicing enhancer (ISE) required for efficient large-intron excision. The ISE binds the cellular RNA-processing proteins TIA-1 and TIAR, which enhance usage of the nonconsensus donor.
FOOTNOTES
- Received 29 January 2009.
- Accepted 24 March 2009.
- ↵*Corresponding author. Mailing address: 471f Life Sciences Center, University of Missouri—Columbia, 1201 Rollins Rd., Columbia, MO 65211. Phone: (573) 882-3920. Fax: (573) 884-9676. E-mail: pinteld{at}missouri.edu
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↵† Present address: Gene Therapy Center, University of North Carolina—Chapel Hill, Chapel Hill, NC 27599-7352.
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↵▿ Published ahead of print on 1 April 2009.
- American Society for Microbiology
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- Article
- ABSTRACT
- U- and A-rich intronic sequences immediately downstream of the 5′ splice site of the large intron are required for its efficient excision.
- The activity of the ISE is distance dependent.
- TIA-1 and TIAR are important for the efficient use of the MVM nonconsensus large-intron donor.
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