Skip to main content
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems
  • Log in
  • My alerts
  • My Cart

Main menu

  • Home
  • Articles
    • Current Issue
    • Accepted Manuscripts
    • COVID-19 Special Collection
    • Minireviews
    • JVI Classic Spotlights
    • Archive
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About JVI
    • Editor in Chief
    • Editorial Board
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • RSS
    • FAQ
  • Subscribe
    • Members
    • Institutions
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems

User menu

  • Log in
  • My alerts
  • My Cart

Search

  • Advanced search
Journal of Virology
publisher-logosite-logo

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Accepted Manuscripts
    • COVID-19 Special Collection
    • Minireviews
    • JVI Classic Spotlights
    • Archive
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About JVI
    • Editor in Chief
    • Editorial Board
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • RSS
    • FAQ
  • Subscribe
    • Members
    • Institutions
Structure and Assembly

The Pseudorabies Virus US3 Protein Is a Component of Primary and of Mature Virions

Harald Granzow, Barbara G. Klupp, Thomas C. Mettenleiter
Harald Granzow
1Institutes of Infectology
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Barbara G. Klupp
2Molecular Biology, Friedrich-Loeffler-Institutes, Federal Research Centre for Virus Diseases of Animals, D-17493 Greifswald-Insel Riems, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Thomas C. Mettenleiter
2Molecular Biology, Friedrich-Loeffler-Institutes, Federal Research Centre for Virus Diseases of Animals, D-17493 Greifswald-Insel Riems, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: mettenleiter@rie.bfav.de
DOI: 10.1128/JVI.78.3.1314-1323.2004
  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading

Article Figures & Data

Figures

  • FIG. 1.
    • Open in new tab
    • Download powerpoint
    FIG. 1.

    Immunoelectron microscopy of primary enveloped PrV virions. Rabbit kidney cells were infected with PrV-Ka and analyzed 14 h after infection by either conventional (A) or immunoelectron microscopy with antisera against the UL31 (B), UL34 (C), UL36 (D), UL37 (E), UL46 (F), UL47 (G), UL48 (H), UL49 (I), or US3 (J) proteins. Bar, 200 nm.

  • FIG. 2.
    • Open in new tab
    • Download powerpoint
    FIG. 2.

    Immunoelectron microscopy of mature extracellular PrV virions. Rabbit kidney cells were infected with PrV-Ka and analyzed 14 h after infection by either conventional (A) or immunoelectron microscopy using antisera against the UL31 (B), UL34 (C), UL36 (D), UL37 (E), UL46 (F), UL47 (G), UL48 (H), UL49 (I), or US3 (J) proteins. Bar, 200 nm.

  • FIG. 3.
    • Open in new tab
    • Download powerpoint
    FIG. 3.

    Specificity of the US3 antiserum. Rabbit kidney cells were infected with PrV-ΔUS3 and analyzed 14 h after infection by either conventional (A) or immunoelectron microscopy using antisera against the US3 (B), UL31 (C), or UL34 (D) proteins. Bar, 200 nm.

  • FIG. 4.
    • Open in new tab
    • Download powerpoint
    FIG. 4.

    US3 is present at the nuclear membrane. Rabbit kidney cells infected with PrV-Ka were analyzed by immunoelectron microscopy using antisera against US3 (A), UL31 (B), or UL34 (C). Bar, 200 nm.

  • FIG. 5.
    • Open in new tab
    • Download powerpoint
    FIG. 5.

    Detection of US3 in cells infected with tegument protein deletion mutants of PrV. Rabbit kidney cells infected with PrV-ΔUL37 (A and B), PrV-ΔUL47 (C and D), PrV-ΔUL48 (E and F), PrV-ΔUL3.5 (G and H), PrV-ΔUL11 (I and J), and PrV-gEIM− (K and L) were analyzed by conventional electron microscopy (A, C, E, G, I, and K) or immunoelectron microscopy with a monospecific anti-US3 serum (B, D, F, H, J, and L). Bars: 500 nm (A, C, E, G, I, and K); 250 nm (B, D, F, H, J, and L).

  • FIG. 6.
    • Open in new tab
    • Download powerpoint
    FIG. 6.

    Protein composition of PrV-ΔUS3 virions. PrV-Ka (lanes 1) and PrV-ΔUS3 (lanes 2) virions were purified by sucrose gradient centrifugation and analyzed in sodium dodecyl sulfate-polyacrylamide gel electrophoresis with 10% (A and C) or 15% (B) polyacrylamide. After electrotransfer onto nitrocellulose membranes, blots were analyzed with antibodies against the US3, UL37, UL46, UL47, UL48, UL49, UL11, gB, gC, gD, gE, gI, gH, gK, and gM proteins. The locations of molecular mass markers are indicated on the left.

  • FIG. 7.
    • Open in new tab
    • Download powerpoint
    FIG. 7.

    US3 protein is a component of PrV-Ba virions. PrV-Ka, PrV-Be, and PrV-Ba virions were purified by sucrose gradient centrifugation and analyzed by immunoblotting with antisera against the US3, UL49, UL37, gE, and gH proteins. The locations of molecular mass markers are indicated on the left.

PreviousNext
Back to top
Download PDF
Citation Tools
The Pseudorabies Virus US3 Protein Is a Component of Primary and of Mature Virions
Harald Granzow, Barbara G. Klupp, Thomas C. Mettenleiter
Journal of Virology Jan 2004, 78 (3) 1314-1323; DOI: 10.1128/JVI.78.3.1314-1323.2004

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Print

Alerts
Sign In to Email Alerts with your Email Address
Email

Thank you for sharing this Journal of Virology article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
The Pseudorabies Virus US3 Protein Is a Component of Primary and of Mature Virions
(Your Name) has forwarded a page to you from Journal of Virology
(Your Name) thought you would be interested in this article in Journal of Virology.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
The Pseudorabies Virus US3 Protein Is a Component of Primary and of Mature Virions
Harald Granzow, Barbara G. Klupp, Thomas C. Mettenleiter
Journal of Virology Jan 2004, 78 (3) 1314-1323; DOI: 10.1128/JVI.78.3.1314-1323.2004
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Top
  • Article
    • ABSTRACT
    • MATERIALS AND METHODS
    • RESULTS
    • DISCUSSION
    • ACKNOWLEDGMENTS
    • FOOTNOTES
    • REFERENCES
  • Figures & Data
  • Info & Metrics
  • PDF

KEYWORDS

Herpesvirus 1, Suid
Viral Proteins
virion
virus assembly

Related Articles

Cited By...

About

  • About JVI
  • Editor in Chief
  • Editorial Board
  • Policies
  • For Reviewers
  • For the Media
  • For Librarians
  • For Advertisers
  • Alerts
  • RSS
  • FAQ
  • Permissions
  • Journal Announcements

Authors

  • ASM Author Center
  • Submit a Manuscript
  • Article Types
  • Ethics
  • Contact Us

Follow #Jvirology

@ASMicrobiology

       

 

JVI in collaboration with

American Society for Virology

ASM Journals

ASM journals are the most prominent publications in the field, delivering up-to-date and authoritative coverage of both basic and clinical microbiology.

About ASM | Contact Us | Press Room

 

ASM is a member of

Scientific Society Publisher Alliance

 

American Society for Microbiology
1752 N St. NW
Washington, DC 20036
Phone: (202) 737-3600

Copyright © 2021 American Society for Microbiology | Privacy Policy | Website feedback

Print ISSN: 0022-538X; Online ISSN: 1098-5514