Article Figures & Data
Figures
- FIG. 1.
Immunoelectron microscopy of primary enveloped PrV virions. Rabbit kidney cells were infected with PrV-Ka and analyzed 14 h after infection by either conventional (A) or immunoelectron microscopy with antisera against the UL31 (B), UL34 (C), UL36 (D), UL37 (E), UL46 (F), UL47 (G), UL48 (H), UL49 (I), or US3 (J) proteins. Bar, 200 nm.
- FIG. 2.
Immunoelectron microscopy of mature extracellular PrV virions. Rabbit kidney cells were infected with PrV-Ka and analyzed 14 h after infection by either conventional (A) or immunoelectron microscopy using antisera against the UL31 (B), UL34 (C), UL36 (D), UL37 (E), UL46 (F), UL47 (G), UL48 (H), UL49 (I), or US3 (J) proteins. Bar, 200 nm.
- FIG. 3.
Specificity of the US3 antiserum. Rabbit kidney cells were infected with PrV-ΔUS3 and analyzed 14 h after infection by either conventional (A) or immunoelectron microscopy using antisera against the US3 (B), UL31 (C), or UL34 (D) proteins. Bar, 200 nm.
- FIG. 4.
US3 is present at the nuclear membrane. Rabbit kidney cells infected with PrV-Ka were analyzed by immunoelectron microscopy using antisera against US3 (A), UL31 (B), or UL34 (C). Bar, 200 nm.
- FIG. 5.
Detection of US3 in cells infected with tegument protein deletion mutants of PrV. Rabbit kidney cells infected with PrV-ΔUL37 (A and B), PrV-ΔUL47 (C and D), PrV-ΔUL48 (E and F), PrV-ΔUL3.5 (G and H), PrV-ΔUL11 (I and J), and PrV-gEIM− (K and L) were analyzed by conventional electron microscopy (A, C, E, G, I, and K) or immunoelectron microscopy with a monospecific anti-US3 serum (B, D, F, H, J, and L). Bars: 500 nm (A, C, E, G, I, and K); 250 nm (B, D, F, H, J, and L).
- FIG. 6.
Protein composition of PrV-ΔUS3 virions. PrV-Ka (lanes 1) and PrV-ΔUS3 (lanes 2) virions were purified by sucrose gradient centrifugation and analyzed in sodium dodecyl sulfate-polyacrylamide gel electrophoresis with 10% (A and C) or 15% (B) polyacrylamide. After electrotransfer onto nitrocellulose membranes, blots were analyzed with antibodies against the US3, UL37, UL46, UL47, UL48, UL49, UL11, gB, gC, gD, gE, gI, gH, gK, and gM proteins. The locations of molecular mass markers are indicated on the left.
- FIG. 7.
US3 protein is a component of PrV-Ba virions. PrV-Ka, PrV-Be, and PrV-Ba virions were purified by sucrose gradient centrifugation and analyzed by immunoblotting with antisera against the US3, UL49, UL37, gE, and gH proteins. The locations of molecular mass markers are indicated on the left.
