Subcellular Localization and Calcium and pH Requirements for Proteolytic Processing of the Hendra Virus Fusion Protein

Cell lines.Vero cells were grown in Dulbecco's modified Eagle's medium (DMEM; Gibco Invitrogen, Carlsbad, Calif.). Human colon carcinoma LoVo cells were purchased from the American Type Culture Collection and maintained in Ham's F12 medium. Fetal bovine serum (FBS; 10%), penicillin, and streptomycin were added to all media.

Plasmid vectors.Vectors containing the Hendra virus F and G genes were kindly provided by Lin-fa Wang (Australian Animal Health Laboratory). The F gene was released from the pUC18 vector by SalI digestion and ligated into XhoI-digested pCAGGS. Similarly, the G gene was excised from the pGEM-7T vector by NsiI and XhoI digestion and ligated into pCAGGS. Both F and G were cloned into pCAGGS in the correct orientation and sequenced to confirm that gene sequence integrity was maintained following subcloning. Robert Lamb (Howard Hughes Medical Institute [HHMI], Northwestern University) generously provided the pCAGGS-SV5 F and -SV5 HN expression vectors.

Antibodies.Antipeptide antibodies (Genemed Custom Peptide Antibody Service, San Francisco, Calif.) were generated to amino acids 526 to 539 within the cytoplasmic tail of the Hendra F protein. SV5 F antipeptide antibodies to amino acids 82 to 96, within the SV5 F₂ subunit, were kindly provided by Robert Lamb (HHMI, Northwestern University).

Expression of F and G (or HN) proteins.F and G (or HN) proteins from Hendra virus and SV5 were transiently expressed by using the mammalian expression vector pCAGGS, which permits high levels of protein expression from a chicken actin promoter (49). Subconfluent monolayers in 35-mm tissue culture dishes were transiently transfected with plasmid DNA by using Lipofectamine (Invitrogen) according to the manufacturer's instructions. Two micrograms of pCAGGS-Hendra F and/or 1 μg of pCAGGS-Hendra G, -SV5 F, or -SV5 HN, 6 μl of Plus reagent, and 4 μl of Lipofectamine in 0.8 ml of OPTI-MEM (Gibco Invitrogen) were combined and added to the monolayers. At 3 h posttransfection, cells were washed twice in phosphate-buffered saline and incubated overnight at 37°C in 2 ml of OPTI-MEM.

Pulse-chase experiments and immunoprecipitation.Following transfection, pulse-chase experiments were performed. Cells were washed and starved for 45 min in DMEM deficient in methionine and cysteine. Tran³⁵S-label (100 μCi/ml; MP Biomedicals, Inc., Irvine, Calif.) was added to the methionine- and cysteine-deficient DMEM, and cells were labeled for 30 min. The labeling medium was removed, cells were washed, and normal DMEM with or without 10% FBS (or 1% bovine serum albumin [BSA]) was added. Cells were then chased for various time intervals. At the end of the chase period, cells were washed twice with phosphate-buffered saline followed by lysis with RIPA buffer containing 100 mM Tris-HCl (pH 7.4), 150 mM NaCl, 0.1% sodium dodecyl sulfate (SDS), 1% Triton X-100, 1% deoxycholic acid, protease inhibitors (1 KalliKrein inhibitory unit of aprotinin [Calbiochem, San Diego, Calif.], 1 mM phenylmethylsulfonyl fluoride [Sigma, St. Louis, Mo.], and Complete protease inhibitor tablets [Roche Molecular Biochemicals, Indianapolis, Ind.]) and 25 mM iodoacetamide (Sigma). The lysates were centrifuged at 136,500 × g for 10 min at 4°C, and supernatants were collected. Antipeptide sera and protein A-conjugated Sepharose beads (Amersham, Piscataway, N.J.) were used to immunoprecipitate the F proteins as previously described (54). Immunoprecipitated F proteins were analyzed via SDS-15% polyacrylamide gel electrophoresis (SDS-PAGE) and visualized using the STORM imaging system (Amersham).

Inhibition of exocytic transport.A number of different chemicals were used to inhibit exocytic transport within the cell. Monensin (20 μM; Sigma) and 5 μg of brefeldin A (Sigma)/ml were present throughout the pulse-chase experiment. Carbonyl cyanide 3-chlorophenylhydrazone (CCCP; 50 μg/ml; Sigma) was added only to the chase medium. A mixture of 30 mM NaF-0.05 mM AlCl₃ · 6H₂O (Sigma) was included in the labeling and chase media. Inhibition of proteolytic cleavage was also examined by chasing F-transfected cells at 20 or 37°C for 2 h, followed by a further chase for 1 h at 37°C. Inhibitor assays used DMEM without FBS for the chase medium.

Cellular Ca²⁺ and pH manipulation assays.Manipulation of intracellular Ca²⁺ concentrations was undertaken by including various concentrations of EGTA (Sigma) and A23187 (Calbiochem) in the label and chase media. For the Ca²⁺ assays, cells were starved and labeled in Ca²⁺-methionine-cysteine-deficient medium (Specialty Media, Phillipsburg, N.J.) and chased with minimal essential medium (Gibco Invitrogen).

Intracellular pH levels were modified by the addition of different concentrations of chloroquine (Sigma), NH₄Cl (Sigma), bafilomycin A1 (Calbiochem), and concanamycin A (Calbiochem). Chloroquine and NH₄Cl were present throughout the starvation, label, and chase periods, whereas bafilomycin A1 and concanamycin A were added only to the chase medium.

Endo H digestion.Endoglycosidase H (Endo H) digestion of immunoprecipitated F proteins was performed as previously described (54). In brief, immunoprecipitated F proteins were boiled for 4 min in 0.4% SDS and 20 mM Na₂HPO₄ (pH 8). Supernatants were collected and incubated with 0.1 M sodium citrate (pH 5.3) and 1 mM phenylmethylsulfonyl fluoride in the absence or presence of 2 mU of Endo H (Roche Molecular Biochemicals) at 37°C for 27 h.

Proteolytic cleavage of the Hendra virus F protein.Synthesis and proteolytic cleavage of the Hendra virus F protein have been observed in several cell lines infected with the virus (44) as well as when the F protein was expressed using a recombinant vaccinia virus expression system (7). To examine the proteolytic processing of the Hendra virus F protein in the absence of viral infection, the Hendra F gene was subcloned into the mammalian expression vector pCAGGS (49). This vector allows for a high level of transient expression from the chicken actin promoter without the requirement of additional plasmid or virus. pCAGGS-Hendra F was transiently transfected into Vero cells, and proteolytic cleavage of the Hendra F protein was monitored by pulse-chase analysis (Fig. 1A). Cleavage of Hendra F protein was examined by immunoprecipitation of the F protein from lysed cells with a Hendra F-specific antibody to the cytoplasmic tail (526 to 539 amino acids), followed by separation on a 15% denaturing polyacrylamide gel and analysis on the STORM imaging system. The empty pCAGGS vector was transfected into Vero cells and used as a control. Immediately after labeling with Tran³⁵S-label (0 h), only F₀ the precursor form was apparent (Fig. 1A, lane 1). Cleavage of the Hendra F₀ precursor to F₁ and F₂ subunits was observed following a 1-h chase, with levels of cleavage increasing during 1 to 4 h. Some F₀ appeared to remain stable in the cell for long periods of time, as a small amount was still immunoprecipitated even after a 24-h chase (Fig. 1A, lane 9). As the analysis of proteolytic processing of the Hendra F protein was conducted over an extended time period (24 h), serum was included in the chase medium to maintain cell viability. Serum contains a plethora of proteins, including proteases and protease inhibitors. To exclude the possibility that serum proteins affected Hendra F₀ cleavage, a truncated pulse-chase analysis (0 to 6 h) in the presence of DMEM and 1% BSA was undertaken. The extent of Hendra F₀ processing and appearance of F₁ and F₂ with serum-free chase medium gave identical results to that observed in Fig. 1A (data not shown), suggesting that serum neither increases nor retards cleavage of the Hendra F protein. As the absence of FBS did not influence cleavage, subsequent experiments to determine the subcellular location and Ca²⁺ and pH requirements of the proteolytic event were performed in the absence of serum to avoid possible sequestration or degradation of the drugs. Additionally, as the majority of cleavage appears to occur within the first 4 h after synthesis and the F₁ and F₂ forms were clearly visible at 2 h after labeling, this time point was used in all subsequent inhibitor experiments. We also examined the expression and processing of the Hendra F protein by using the recombinant vaccinia virus-T7 RNA polymerase transient-expression system by pulse-chase analysis. Proteolytic cleavage of the Hendra F protein was not apparent until 6 h after labeling, with maximal cleavage observed at 12 h (data not shown), indicating that the pCAGGS expression system was a more appropriate system for study of proteolytic activation of the Hendra F protein.

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FIG. 1.

Expression of the Hendra F protein. (A) Vero cells transfected with pCAGGS-Hendra F (2 μg) were starved for 45 min, labeled with 100 μCi of Tran³⁵S-label/ml for 30 min, and either lysed immediately (0 h) or chased in DMEM with 10% FBS for 1, 2, 4, 6, 9, 12, and 24 h prior to lysis. Samples were immunoprecipitated, separated on an SDS-15% polyacrylamide gel, and analyzed by using the STORM imaging system. (B) Vero cells were cotransfected with pCAGGS-Hendra F (2 μg) and empty pCAGGS vector (1 μg); pCAGGS-Hendra F (2 μg) and pCAGGS-Hendra G (1 μg); or pCAGGS-Hendra F (2 μg) and pCAGGS-SV5 HN (1 μg). Transfected cells were metabolically labeled and chased in DMEM and 1% BSA for 0, 1, 2, 4, and 6 h. Samples were analyzed as before by immunoprecipitation, SDS-15% PAGE, and the STORM imaging system. The arrows on the right designate the positions of the F₀, F₁, and F₂ proteins.

The presence of Hendra G, the attachment protein, is required for Hendra F-mediated membrane fusion (7). To determine whether Hendra G (the only other viral glycoprotein within the secretory pathway) enhanced proteolytic processing of the Hendra F protein, Hendra F and Hendra G were coexpressed in Vero cells and cleavage was examined over a 6-h time period. The empty pCAGGS vector as well as the SV5 attachment protein (HN) were cotransfected with Hendra F (in place of Hendra G) in parallel experiments as negative controls. The coexpression of either Hendra G or SV5 HN did not influence the cleavage of Hendra F or the amount of F protein processed (Fig. 1B), suggesting that the factor(s) needed for cleavage is present in uninfected Vero cells.

Furin does not proteolytically process the Hendra F protein.An aberrant nucleotide deletion within the P domain of the furin gene in LoVo cells renders furin functionally inactive (60). The Hendra virus F protein lacks a furin cleavage motif, and infectious virus has been successfully propagated in LoVo cells (44). In order to compare the expression of Hendra F in furin-positive and -negative cell lines, pCAGGS-Hendra F was transfected into LoVo cells and cleavage was monitored over a 24-h time course by pulse-chase analysis. Similar to proteolytic processing of Hendra F in Vero cells, precursor F₀ was observed immediately following metabolic labeling (0 h) (Fig. 2, lane 1), and cleavage to the F₁ and F₂ disulfide-linked forms similarly occurred during the chase period (Fig. 2, lanes 2 to 7). Overall expression levels in LoVo cells were decreased, making detection of the F₂ subunit more difficult. The similar kinetics of proteolytic processing of the Hendra F protein in this cell line confirmed that furin is not involved in proteolytically cleaving Hendra F.

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FIG. 2.

The Hendra F protein is proteolytically processed in LoVo cells. LoVo cells were transiently transfected with pCAGGS-Hendra F (2 μg), metabolically labeled, and lysed immediately (0 h) or chased in DMEM and 10% FBS for various times prior to lysis. Samples were immunoprecipitated and analyzed via SDS-15% PAGE and by the STORM imaging system. The F₀ and F₁ proteins are marked with arrows on the right.

Inhibitors of exocytic transport abolish proteolytic processing of the Hendra F protein.Infectious Hendra virus has been successfully propagated in different cell lines in the absence of exogenous trypsin (44, 48), indicating that an extracellular protease that cleaves at the basic residue is not required. Additionally, it has been established that the Hendra F protein is not proteolytically processed by the secretory protease furin. To determine the subcellular location of Hendra F protein processing, pCAGGS-Hendra F-transfected Vero cells were metabolically labeled and incubated in the absence or presence of compounds known to affect exocytic transport. To examine whether proteolytic processing of the Hendra F protein occurs within the ER, an uncoupler of oxidative phosphorylation, CCCP, which is known to block the transport of proteins from the ER (15), was included in the chase medium. Cleavage of the Hendra F₀ protein to generate F₁ and F₂ subunits was not inhibited in the presence of dimethyl sulfoxide alone (Fig. 3, lane 2); however, proteolytic processing of the Hendra F protein was completely blocked following incubation of 50 μg of CCCP/ml in dimethyl sulfoxide (Fig. 3, lane 3). These results demonstrate that cleavage of the Hendra F protein does not occur in the ER.

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FIG. 3.

Inhibitors of vesicular transport block proteolytic processing of the Hendra F protein. Vero cells transfected with pCAGGS-Hendra F (2 μg) were starved for 45 min, labeled with Tran³⁵S-label, and immediately lysed or chased for 2 h in DMEM in the absence (−) or presence (+) of 50 μg of CCCP/ml, 20 μM monensin, and 5 μg of brefeldin A/ml. Monensin and brefeldin A were included in the starvation, label, and chase media, while CCCP was present only in the chase medium. Samples were immunoprecipitated, separated on an SDS-15% polyacrylamide gel, and analyzed by use of the STORM imaging system. The uncleaved and cleaved forms of the Hendra F protein are designated with arrows on the right.

Vesicular transport between the medial and trans-Golgi cisternae is inhibited by the sodium ionophore monensin (26). Therefore, to determine whether proteolytic processing of the Hendra F protein occurred in the early compartments of the Golgi stack, pCAGGS-Hendra F-transfected Vero cells were metabolically labeled and chased for 2 h in the absence or presence of 20 μM monensin. Monensin was present throughout the pulse-chase experiment. Hendra F protein cleavage occurred in the presence of methanol (control) (Fig. 3, lane 4) but was completely abolished in the presence of monensin (in methanol) (Fig. 3, lane 5). Proteolytic cleavage of the Hendra F protein was also monitored by Endo H digestion. Endo H specifically digests high-mannose carbohydrates. In the medial Golgi, modifications and additions to high-mannose carbohydrates produce complex carbohydrates refractory to digestion by Endo H (54). Therefore, Endo H processing is often used as a marker of vesicular transport. Vero cells transfected with pCAGGS-Hendra F were metabolically labeled and chased for 0, 0.5, 1, 2, and 6 h. Endo H digestion of immunoprecipitated Hendra F protein showed that F₀ became Endo H resistant prior to the cleavage event (data not shown). The inhibition of cleavage in the presence of monensin as well as Endo H resistance prior to cleavage suggest that protease activity resides in a compartment distal to the medial Golgi.

The fungal metabolite brefeldin A prevents anterograde trafficking from the ER while maintaining retrograde transport from the cis-, medial, and trans-Golgi (17, 40). The effect of brefeldin A on Hendra F cleavage was examined by the incorporation of brefeldin A in methanol (5 μg/ml) into the starvation, label, and chase media of pCAGGS-Hendra F-transfected Vero cells. In the presence of brefeldin A, Hendra F protein proteolytic processing was prevented (Fig. 3, lane 7). These data suggest that either redistributed enzymes, as a result of the Golgi stack collapse, were nonfunctional within the ER environment or that cleavage of the Hendra F protein occurs in the TGN or at the cellular surface. As the presence of each of these vesicular transport inhibitors abolished proteolytic cleavage of the Hendra F protein, processing occurs in the TGN or at the cell surface.

Similar to many paramyxoviruses, the SV5 F protein contains a polybasic cleavage motif and is known to be processed by furin (20) in the TGN. As a control, the effects of each of these inhibitors on proteolytic cleavage of the SV5 F protein were studied. CCCP, monensin, and brefeldin A similarly disrupted cleavage of the SV5 F protein, consistent with cleavage in the TGN (data not shown).

Cleavage of the Hendra F protein occurs either in the secretory vesicles or at the cell surface.The formation of secretory vesicles from the trans-Golgi has been shown to be inhibited by the treatment of cells with a mixture of NaF and AlCl₃ (3). To analyze whether proteolytic processing of the Hendra F protein occurs before or after the formation of secretory vesicles in the TGN, Vero cells transiently expressing Hendra F or SV5 F (as a positive control) were metabolically labeled and chased for 2 h as described previously. The 30 mM NaF-0.05 mM AlCl₃ mixture was included in the label and chase periods. Although the presence of NaF-AlCl₃ did not affect expression of the Hendra and SV5 F₀ protein, cleavage to the F₁ and F₂ forms was prevented (Fig. 4A, lanes 4 and 8). We noted with SV5 F that immediately after labeling with Tran³⁵S-label, a small amount of cleavage had already occurred. Additionally, a second band migrating slightly ahead of F₁ was visible, although this band was no longer visible following the 2-h chase in DMEM. This additional band may represent either a degraded form of the SV5 F product or a labeled cellular protein that is subsequently degraded during the 2-h chase.

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FIG. 4.

Proteolytic processing of Hendra F occurs proximal to the trans-Golgi. (A) Vero cells were transfected with pCAGGS-Hendra F (2 μg) and -SV5 F (1 μg) expression plasmids, metabolically labeled with Tran³⁵S-label, chased for 2 h in DMEM in the absence (−) or presence (+) of 30 mM NaF-0.05 mM AlCl₃, and analyzed by SDS-15% PAGE and the STORM imaging system. The NaF-AlCl₃ mixture was included in the labeling and chase media. (B) Vero cells were transfected with pCAGGS-Hendra F (2 μg) and -SV5 F (1 μg), metabolically labeled, and chased for 2 h at either 37 or 20°C, followed by an additional 1-h incubation at 37°C. Samples were immunoprecipitated and analyzed as described above. The arrows on the right designate the positions of the F₀, F₁, and F₂ proteins.

Temperature block experiments have been useful in examining trafficking of secretory and membrane proteins. Incubation of cells at 20°C influences both exocytic (43) and endocytic transport (25). Budding of immature secretory vesicles from the TGN and transport of newly synthesized proteins to the plasma membrane are prevented when cells are incubated at 20°C (43). Therefore, a temperature shift assay was undertaken to confirm results from the NaF-AlCl₃ experiment. Vero cells transfected with pCAGGS-Hendra F and SV5 F (as a control) were metabolically labeled as before. Following labeling, transfected cells were chased either at 20 or 37°C for 2 h. Cleaved Hendra and SV5 F products were clearly visible following the 2-h chase at 37°C (Fig. 4B, lanes 2 and 7). In contrast, proteolytic processing of both Hendra and SV5 F₀ was blocked when cells were chased at 20°C (Fig. 4B, lanes 4 and 9). However, when cells were shifted back to 37°C for an additional hour, restoring budding and transport of secretory vesicles, cleavage was observed (Fig. 4B, lanes 5 and 10). Inhibition of SV5 F proteolysis at 20°C is consistent with cleavage by furin occurring in the secretory vesicles budding from the TGN. Thus, proteolytic processing of the Hendra F protein was inhibited by both NaF-AlCl₃ and a 20°C temperature shift, indicating that cleavage occurred either in the secretory vesicles budding from the TGN or at the cell surface.

A decrease in intracellular Ca²⁺ levels does not significantly influence proteolytic processing of the Hendra F protein.Changes in intracellular Ca²⁺ have been shown to influence the proteolytic activity of cellular secretory proteases, such as furin and other members of the PC family, including the ER protease SKI-1/S1P (56, 57). Therefore, the effect on cleavage of Hendra F following changes in intracellular Ca²⁺ levels was examined. pCAGGS-Hendra F-transfected and SV5 F-transfected (as a positive control) Vero cells were metabolically labeled, and Ca²⁺ levels were depleted by incubation during the label and chase periods with various concentrations of EGTA in Ca²⁺-deficient medium. Consistent with the Ca²⁺ dependence of furin, incubation with EGTA significantly reduced the processing of SV5 F, even at concentrations as low as 0.1 mM (Fig. 5B, lane 3). In contrast, the addition of EGTA up to 5 mM did not significantly affect cleavage of the Hendra F protein (Fig. 5A, lanes 3 to 7). A slight decrease in molecular weight of the Hendra F₂ protein was also observed (Fig. 5A, lanes 3 to 7). Changes in intracellular Ca²⁺ levels may influence carbohydrate modifications (42) and, thus, may explain the molecular weight shift. Cleavage of the Hendra F protein was also analyzed in the presence of various concentrations of the Ca²⁺ ionophore A23187 (0.1 to 5 μM) (Fig. 5C). A23187 similarly reduced the amount of cleavage of the SV5 F protein (Fig. 5D, lanes 3 to 7) but did not significantly perturb proteolytic processing of the Hendra F protein (Fig. 5C, lanes 3 to 7). Cleavage of Hendra F was slightly reduced at the highest A23187 concentrations (Fig. 5C, lanes 6 and 7). This decrease in proteolytic processing may either be due to a toxic effect of A23187 at such concentrations or may indicate that the Hendra F protein cleavage event is not completely independent of Ca²⁺. Similar to experiments performed in the presence of EGTA, the molecular weight of the Hendra F₂ subunit decreased following the addition of A23187 (Fig. 5C, lanes 3 to 7). Thus, manipulations of intracellular Ca²⁺ do not significantly alter proteolytic processing of the Hendra F protein, suggesting that the protease involved in the processing of Hendra F appears to have a decreased Ca²⁺ requirement compared to that of furin or SKI-1/S1P.

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FIG. 5.

A decrease in calcium does not significantly affect proteolytic cleavage of the Hendra F protein. Vero cells were transfected with pCAGGS-Hendra F (2 μg; A and C) or -SV5 F (1 μg; B and D) expression plasmids and metabolically labeled in the absence (−) or presence (+) of increasing concentrations of EGTA (A and B) or A23187 (C and D). EGTA and A23187 were added to label and chase media, and calcium-free media were used throughout the experiment. Samples were immunoprecipitated and analyzed as described before. Precursor F₀ and cleaved F₁ and F₂ subunits are indicated by the arrows on the right.

Proteolytic processing of Hendra virus F is inhibited by changes in intracellular pH.The pH within subcompartments of the secretory pathway is thought to play an important role in their normal functioning (2). Proteins undergoing transport through the secretory pathway from the ER to TGN encounter pH changes from 7.1 to 5.91 (16, 36, 41). Our data suggest that the Hendra F protein is proteolytically processed either in the secretory vesicles budding from the TGN or at the cell surface, two locations with remarkably different pHs. It was therefore of interest to examine whether manipulations of intracellular pH affected cleavage of Hendra F. Weak basic amines such as chloroquine and NH₄Cl (32, 50), as well as inhibitors of the vacuolar H⁺ V-ATPase, bafilomycin A1 and concanamycin A (18, 41), have been shown to raise intracellular pH. Vero cells transiently expressing Hendra F and SV5 F were metabolically labeled in the presence of increasing concentrations of chloroquine (1 to 100 μM), NH₄Cl (1 to 20 mM), or bafilomycin A1 or concanamycin A (10 to 100 nM). Furin is known to be proteolytically active in the TGN and at the cell surface, as well as in endocytic vesicles, and has been characterized to be active over a broad pH range (pH 6 to 8) (62). The finding that increasing concentrations of chloroquine did not inhibit cleavage of the SV5 F protein was thus expected (Fig. 6B, lanes 3 to 6), with reduction only seen at 100 μM chloroquine (Fig. 6B, lane 7). At this concentration, chloroquine may have influenced initial synthesis and folding of SV5 F in the ER, reduced or abolished vesicular transport, or adversely affected the proteolytic activity of furin. Surprisingly, chloroquine concentrations greater than 1 μM abolished proteolytic processing of the Hendra F protein (Fig. 6A, lanes 3 to 7). As seen with SV5 F, the overall amount of Hendra F₀ decreased in the presence of 100 μM chloroquine (Fig. 6A, lane 7), similarly suggesting that at this high concentration initial synthesis and folding of Hendra F in the ER may be retarded. NH₄Cl (10 mM), bafilomycin A1 (50 nM), and concanamycin A (10 nM) similarly inhibited cleavage of Hendra F but not that of SV5 F (data not shown). Inhibition of Hendra F₀ cleavage could potentially be due to the accumulation of F₀ in either the ER or Golgi complex. To exclude this possibility, vesicular trafficking of Hendra F following pH manipulations was monitored by Endo H digestion. As can be seen in Fig. 6C (lanes 1 to 4), Endo H digestion of Hendra F from untreated cells immediately after labeling and the 2-h chase period permitted identification of Endo H-sensitive and -resistant F₀ (F_o^s and F_o^r) and F₁ (F₁^r) forms. Endo H-resistant Hendra F₀ following treatment with 10 μM chloroquine, 50 nM bafilomycin A, and 10 nM concanamycin A (Fig. 6C, lanes 6, 10, and 12, respectively) was identified, suggesting that the Hendra F protein was efficiently transported through the medial Golgi. In contrast, treatment with 10 mM NH₄Cl retarded exocytic transport, as a predominantly Endo H-sensitive form of Hendra F₀ was identified (Fig. 6C, lane 8). Therefore, changes in intracellular pH inhibit proteolytic processing of Hendra F, and this effect is not due to a blockage of exocytic transport.

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FIG. 6.

Changes in intracellular pH retard proteolytic processing of Hendra F but not the SV5 F protein. Vero cells were transfected with pCAGGS-Hendra F (2 μg; A) or -SV5 F (1 μg; B) expression plasmids, metabolically labeled, and chased for 2 h in DMEM. Increasing concentrations of chloroquine were present in the starvation, label, and chase media. Cells were lysed, immunoprecipitated, and analyzed by SDS-15% PAGE and the STORM imaging system. The arrows on the right designate the positions of the F₀, F₁, and F₂ proteins. (C) pCAGGS-Hendra F-transfected Vero cells were metabolically labeled and chased as before in the absence (−) or presence (+) of 10 μM chloroquine, 10 mM NH₄Cl, 50 nM bafilomycin A1, or 10 nM concanamycin A. Chloroquine and NH₄Cl were present in the starvation, label, and chase media, and bafilomycin A1 and concanamycin A were present only in the chase medium. Samples were lysed, immunopreciptated, and either mock treated (−) or treated with 2 mU of Endo H (+) for 27 h at 37°C prior to analysis by SDS-10% PAGE and the STORM imaging system. Endo H-sensitive and -resistant F₀ (F₀^s and F_o^r) and F₁ (F₁^r) forms are indicated by the arrows on the right.