Article Figures & Data
Figures
- FIG. 1.
Construction of recombinant virus. Below the top two lines, which represent the tkLTRZ1 genome and the location of the tk gene (UL23), is a schematic diagram of the functional domains of TK and the location of the G string. The bottom four lines show the wild-type nucleotide sequence and a three-frame translation of this sequence, with the wild-type frame marked as the 0 frame.
- FIG. 2.
(A) Semiquantitative plaque autoradiography of viruses. The top line of values shows the approximate amounts of active TK expressed by each mutant as a percentage of that expressed by the wild-type strain KOS. The next line shows the average amount of radioactivity measured per plaque as a percentage relative to that measured for KOS. The next line presents the images of the plates. The relative level of TK activity associated with TKG7+2G is shown above the image of the plate used to ascertain this value. Relative TK activity levels were estimated from a graph (data not shown) of the relative percentage of TK polypeptide plotted against the relative percentage of TK activity in situ for LS-111/-101//-56/-46, 615.9, LS-29/-18, and tkLTRZ1 (y = 0.034x + 0.2684; R2 = 0.91). The arrowhead on the TKG7+2G autoradiograph indicates a plaque with TK+ activity. (B) Enlarged image of a single plaque that appears to have a mixed phenotype. The white arrowhead indicates a cell with a low level of TK activity, and the black arrowhead indicates a cell with a high level of TK activity.
- FIG. 3.
Plaque autoradiography of viruses isolated during the acute phase of infection with TKG7+2G. The percentage of plaques with high levels of TK activity among those with low levels of TK activity is noted. In each image, one example each of a plaque with a high level of TK activity and a TKL plaque is indicated with a black and a white arrowhead, respectively.
- FIG. 4.
Plaque autoradiography of viruses isolated following reactivation of TKG7+2G. In the image of the mixed population, one example each of a plaque with a high level of TK activity and a TKL plaque is indicated with a black and a white arrowhead, respectively.
Tables
- TABLE 1.
Virus titers in eye swabs and ganglia during acute infections of mice
Virus Titer (log meana ± SE) at indicated location and time p.i.b Eye Trigeminal ganglia Day 1 Day 2 Day 3 Day 3 KOS 4.3 ± 0 (3) 5.1 ± 0.2 (4) 4.3 ± 0.3 (4) 5.3 ± 0.3 (3) tkLTRZ1 5.4 ± 0.2 (3) 4.0 ± 0.3 (4) 2.6 ± 0.2 (4) 0.3 ± 0 (4) TKG7+2G.1 5.6 ± 0.5 (2) 5.5 ± 0.2 (2) 2.3 ± 0.2 (2) 3.9 ± 0.2 (2) TKG7+2G.2 4.6 (1) 4.4 ± 0 (2) 3.5 ± 0.3 (2) 3.8 ± 0.3 (2)
- Top
- Article
- ABSTRACT
- Construction of recombinant virus TKG7+2G.
- Heterogeneity of TK activity of virus carrying a double-G insertion.
- Enrichment of virus with high levels of TK activity during acute replication in the mouse.
- Reactivation from latency.
- What accounts for the low level of TK activity of most TKG7+2G plaques?
- What causes the high frequency of phenotypic reversion?
- ACKNOWLEDGMENTS
- FOOTNOTES
- REFERENCES
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