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Pathogenesis and Immunity

A Lymphatic Mechanism of Rotavirus Extraintestinal Spread in the Neonatal Mouse

Eric C. Mossel, Robert F. Ramig
Eric C. Mossel
Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas 77030
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Robert F. Ramig
Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas 77030
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  • For correspondence: rramig@bcm.tmc.edu
DOI: 10.1128/JVI.77.22.12352-12356.2003
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  • FIG. 1.
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    FIG. 1.

    Virus titers in various tissues following oral inoculation of neonatal mice with (A) RRV, (B) SA11-Cl4, (C) R7, or (D) S7. Five-day-old mice were orally inoculated with 2 × 106 PFU of rotavirus. At the postinoculation times indicated, animals were sacrificed, tissues were harvested, and virus titers were determined by plaque assay. Values indicate mean titers of virus-positive samples. Error bars indicate standard deviations. Gut, large and small intestine less a 2-cm section of terminal ileum; PP, 2-cm section of Peyer's patch-rich terminal ileum. Results for RRV and SA11-Cl4 at 6 hpi and for R7 and S7 are from 5 to 6 animals. Results for RRV and SA11-Cl4 at 12 to 72 hpi are from 9 to 11 animals.

  • FIG. 2.
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    FIG. 2.

    Frequency of virus recovery in various tissues following oral inoculation of neonatal mice with (A) RRV, (B) SA11-Cl4, (C) R7, or (D) S7. Five-day-old mice were orally inoculated with 2 × 106 PFU of rotavirus. At the postinoculation times indicated, animals were sacrificed, tissues were harvested, and virus presence was determined by plaque assay. Gut = large and small intestine less 2 cm section of terminal ileum; PP = 2 cm section of Peyer's patch-rich terminal ileum; MLN = mesenteric lymph nodes. Results for RRV and SA11-Cl4 at 6 hpi and for R7 and S7 are from 5 to 6 animals. Results for RRV and SA11-Cl4 at 12 to 72 hpi are from 9 to 11 animals.

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  • TABLE 1.

    Frequency of detection in the MLN of RRV and SA11-Cl4 reassortant viruses following the oral inoculation of neonatal mice

    VirusnaMLN growthbMLN titercParental origin of genome segmentd (gene product)
    1 (VP1)2 (VP2)3 (VP3)4 (VP4)5 (NSP1)6 (VP6)7 (NSP3)8 (NSP2)9 (VP7)10 (NSP4)11 (NSP5)
    RRV17100%2.8RRRRRRRRRRR
    SA11-Cl4170%0.0SSSSSSSSSSS
    R71080%3.7SSSSSSRSSSS
    S1, 2, 3, 5, 71777%3.1SSSRSRSRRRR
    S71060%3.8RRRRRRSRRRR
    S4, 5, 7, 91855%3.3RRRSSRSRSRR
    R4, 6, 91729%1.2SSSRSRSSRSS
    R91614%0.7SSSSSSSSRSS
    R41812%0.9SSSRSSSSSSS
    R8, 9170%0.0SSSSSSSRRSS
    R10170%0.0SSSSSSSSSRS
    R11180%0.0SSSSSSSSSSR
    • ↵ a n, the number of individual pups inoculated with each virus.

    • ↵ b MLN growth is measured as the percentage of animals in which virus was recovered from the MLN at 3 days postinoculation.

    • ↵ c Titers are the mean titers of the virus in virus-positive MLN at 3 days postinoculation.

    • ↵ d RRV-derived genome segment numbers have been converted to SA11 functional equivalents.

  • TABLE 2.

    Statistical analysis of the contribution of single genome segments to spread of reassortant viruses to the mesenteric lymph node

    AnalysisP for genome segment
    1234567891011
    Student’s t test0.100.100.100.13NAa 0.001 NAb0.050.360.050.05
    Wilcoxon ranked-sum test0.180.180.180.110.33 0.02 NAb0.170.260.170.17
    • ↵ a Statistical analysis by Student’s t test was not applicable (NA), since only one reassortant virus examined contained RRV-derived segment 5.

    • ↵ b Statistical analysis was NA, since all reassortant viruses examined contained SA11-C14-derived segment 7.

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A Lymphatic Mechanism of Rotavirus Extraintestinal Spread in the Neonatal Mouse
Eric C. Mossel, Robert F. Ramig
Journal of Virology Oct 2003, 77 (22) 12352-12356; DOI: 10.1128/JVI.77.22.12352-12356.2003

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A Lymphatic Mechanism of Rotavirus Extraintestinal Spread in the Neonatal Mouse
Eric C. Mossel, Robert F. Ramig
Journal of Virology Oct 2003, 77 (22) 12352-12356; DOI: 10.1128/JVI.77.22.12352-12356.2003
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  • Top
  • Article
    • ABSTRACT
    • Kinetics of rotavirus extraintestinal spread.
    • VP6 is a secondary determinant of extraintestinal spread.
    • ACKNOWLEDGMENTS
    • FOOTNOTES
    • REFERENCES
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KEYWORDS

Antigens, Viral
Lymph Nodes
rotavirus

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