Article Figures & Data
Figures
- FIG. 1.
Virus titers in various tissues following oral inoculation of neonatal mice with (A) RRV, (B) SA11-Cl4, (C) R7, or (D) S7. Five-day-old mice were orally inoculated with 2 × 106 PFU of rotavirus. At the postinoculation times indicated, animals were sacrificed, tissues were harvested, and virus titers were determined by plaque assay. Values indicate mean titers of virus-positive samples. Error bars indicate standard deviations. Gut, large and small intestine less a 2-cm section of terminal ileum; PP, 2-cm section of Peyer's patch-rich terminal ileum. Results for RRV and SA11-Cl4 at 6 hpi and for R7 and S7 are from 5 to 6 animals. Results for RRV and SA11-Cl4 at 12 to 72 hpi are from 9 to 11 animals.
- FIG. 2.
Frequency of virus recovery in various tissues following oral inoculation of neonatal mice with (A) RRV, (B) SA11-Cl4, (C) R7, or (D) S7. Five-day-old mice were orally inoculated with 2 × 106 PFU of rotavirus. At the postinoculation times indicated, animals were sacrificed, tissues were harvested, and virus presence was determined by plaque assay. Gut = large and small intestine less 2 cm section of terminal ileum; PP = 2 cm section of Peyer's patch-rich terminal ileum; MLN = mesenteric lymph nodes. Results for RRV and SA11-Cl4 at 6 hpi and for R7 and S7 are from 5 to 6 animals. Results for RRV and SA11-Cl4 at 12 to 72 hpi are from 9 to 11 animals.
Tables
- TABLE 1.
Frequency of detection in the MLN of RRV and SA11-Cl4 reassortant viruses following the oral inoculation of neonatal mice
Virus na MLN growthb MLN titerc Parental origin of genome segmentd (gene product) 1 (VP1) 2 (VP2) 3 (VP3) 4 (VP4) 5 (NSP1) 6 (VP6) 7 (NSP3) 8 (NSP2) 9 (VP7) 10 (NSP4) 11 (NSP5) RRV 17 100% 2.8 R R R R R R R R R R R SA11-Cl4 17 0% 0.0 S S S S S S S S S S S R7 10 80% 3.7 S S S S S S R S S S S S1, 2, 3, 5, 7 17 77% 3.1 S S S R S R S R R R R S7 10 60% 3.8 R R R R R R S R R R R S4, 5, 7, 9 18 55% 3.3 R R R S S R S R S R R R4, 6, 9 17 29% 1.2 S S S R S R S S R S S R9 16 14% 0.7 S S S S S S S S R S S R4 18 12% 0.9 S S S R S S S S S S S R8, 9 17 0% 0.0 S S S S S S S R R S S R10 17 0% 0.0 S S S S S S S S S R S R11 18 0% 0.0 S S S S S S S S S S R ↵a n, the number of individual pups inoculated with each virus.
↵b MLN growth is measured as the percentage of animals in which virus was recovered from the MLN at 3 days postinoculation.
↵c Titers are the mean titers of the virus in virus-positive MLN at 3 days postinoculation.
↵d RRV-derived genome segment numbers have been converted to SA11 functional equivalents.
- TABLE 2.
Statistical analysis of the contribution of single genome segments to spread of reassortant viruses to the mesenteric lymph node
Analysis P for genome segment 1 2 3 4 5 6 7 8 9 10 11 Student’s t test 0.10 0.10 0.10 0.13 NAa 0.001 NAb 0.05 0.36 0.05 0.05 Wilcoxon ranked-sum test 0.18 0.18 0.18 0.11 0.33 0.02 NAb 0.17 0.26 0.17 0.17
