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REPLICATION

Intrinsic Stability of Episomal Circles Formed during Human Immunodeficiency Virus Type 1 Replication

Theodore C. Pierson, Tara L. Kieffer, Christian T. Ruff, Christopher Buck, Stephen J. Gange, Robert F. Siliciano
Theodore C. Pierson
1Department of Medicine, Johns Hopkins University School of Medicine
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Tara L. Kieffer
1Department of Medicine, Johns Hopkins University School of Medicine
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Christian T. Ruff
1Department of Medicine, Johns Hopkins University School of Medicine
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Christopher Buck
1Department of Medicine, Johns Hopkins University School of Medicine
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Stephen J. Gange
2Department of Epidemiology, Johns Hopkins University School of Public Health, Baltimore, Maryland 21205
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Robert F. Siliciano
1Department of Medicine, Johns Hopkins University School of Medicine
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  • For correspondence: rsilicia@welch.jhu.edu
DOI: 10.1128/JVI.76.8.4138-4144.2002
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ABSTRACT

The development of surrogate markers capable of detecting residual ongoing human immunodeficiency virus type 1 (HIV-1) replication in patients receiving highly active antiretroviral therapy is an important step in understanding viral dynamics and in developing new treatment strategies. In this study, we evaluated the utility of circular forms of the viral genome for the detection of recent infection of cells by HIV-1. We measured the fate of both one-long terminal repeat (1-LTR) and 2-LTR circles following in vitro infection of logarithmically growing CD4+ T cells under conditions in which cell death was not a significant contributing factor. Circular forms of the viral genome were found to be highly stable and to decrease in concentration only as a function of dilution resulting from cell division. We conclude that these DNA circles are not intrinsically unstable in all cell types and suggest that the utility of 2-LTR circle assays in measuring recent HIV-1 infection of susceptible cells in vivo needs to be reevaluated.

  • Copyright © 2002 American Society for Microbiology
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Intrinsic Stability of Episomal Circles Formed during Human Immunodeficiency Virus Type 1 Replication
Theodore C. Pierson, Tara L. Kieffer, Christian T. Ruff, Christopher Buck, Stephen J. Gange, Robert F. Siliciano
Journal of Virology Apr 2002, 76 (8) 4138-4144; DOI: 10.1128/JVI.76.8.4138-4144.2002

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Intrinsic Stability of Episomal Circles Formed during Human Immunodeficiency Virus Type 1 Replication
Theodore C. Pierson, Tara L. Kieffer, Christian T. Ruff, Christopher Buck, Stephen J. Gange, Robert F. Siliciano
Journal of Virology Apr 2002, 76 (8) 4138-4144; DOI: 10.1128/JVI.76.8.4138-4144.2002
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KEYWORDS

DNA, Circular
HIV Infections
HIV-1
Plasmids
virus replication

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