Article Figures & Data
Figures
- FIG. 1.
Detection of RRV, SA11, and Wa antigens in primary NOD mouse islets by indirect immunofluorescence assay after 1 to 5 days of infection at an MOI of 1.0. Rotavirus-infected and uninfected cells were fixed and stained at various times p.i. RRV-infected cells are shown at 24 h (A and D), 72 h (B), and 120 h (C and F) p.i.; uninfected (control) cells are shown at 72 h p.i. (E); SA11-infected cells are shown at 24 h (G) and 48 h (H) p.i.; and Wa-infected cells are shown at 24 h p.i. (I). Infected cells were visualized by fluorescence microscopy (A, B, C, E, G, H, and I). The position of RRV-infected cells within the islet was located by phase-contrast microscopy (D and F). Magnification, ×200.
- FIG. 2.
Relative growth of RRV in primary NOD mouse islets, enterocytes, and MA104 cells up to 72 h p.i. at an MOI of 1.0. Enterocytes were isolated from the same mice from which the pancreatic cells were obtained, as described previously (36), but were infected immediately, without culturing in vitro. Each bar represents the 95% confidence interval of the mean of three replicates.
- FIG. 3.
Kinetics of SA11 growth over 7 days in primary NOD mouse islets at MOIs of 0.1 and 1. The growth curve of SA11 in MA104 cells at an MOI of 1 is included for comparison. Each bar represents the 95% confidence interval of the mean of replicates in two experiments.
- FIG. 4.
Kinetics of RRV growth over 7 days in primary NOD and NOR mouse islets and islet cell-depleted pancreatic cells at MOIs of 0.1 (A) and 1 (B). Growth curves of RRV at the same MOI in MA104 cells are included for comparison. Each bar represents the 95% confidence interval of the mean of replicates obtained in two to four experiments.
- FIG. 5.
Kinetics of growth of human and monkey rotaviruses at an MOI of 1 in primary adult monkey islets. Strain Wa replication in monkey islets is compared with titers of strain Wa in primary NOD mouse islets (A). Growth curves of rotavirus strains RV-5, RRV, and SA11 in primary adult monkey islets are shown in panel B. Each bar represents the standard deviation of the mean of three replicates from each of two experiments.
Tables
- TABLE 1.
Maximum rotavirus titers and maximum increases in virus titers over input produced by monkey and human rotaviruses in cultures of primary islet cells from mice, pigs, and monkeys
Rotavirus strain MOI Maximum virus titer in FCFU/ml (fold increase in titer over inputa) in given islet cell type: Mouse Pig Monkeyb NOD NOR RRVc 0.1 1.7 × 107 (4,570) 1.9 × 106 (503) NDg ND 1 7.0 × 106 (269) 2.6 × 106 (125) ND 3.9 × 104 (23) SA11d 0.1 1.0 × 106 (1,300) ND ND ND 0.5-1 5.2 × 105 (140) ND 8.4 × 104 (40) 1.7 × 104 (20) 10 1.3 × 105 (4.9) ND 6.5 × 106 (3,170) ND Wae 1 4.2 × 103 (1.2) ND ND 6.0 × 103 (6.0) RV-5f 0.5-1 ND ND 3.8 × 103 (1.0) 4.4 × 103 (3.3) ↵ a Input titer is defined as the titer of cell-associated virus at 1 h p.i.
↵ b Maximum virus titers of all rotaviruses were reached at 72 h p.i. in monkey islets.
↵ c Maximum RRV titers were reached at 120 h p.i. in NOD and NOR mouse islets.
↵ d Maximum SA11 titers were reached at 168 h p.i. in NOD mouse islets and at 24 h in pig islet cells.
↵ e Maximum Wa titers were reached at 24 h p.i. in NOD mouse islets.
↵ f Maximum RV-5 titers were reached at 24 h p.i. in pig islet cells.
↵ g ND, not done.
- Top
- Article
- ABSTRACT
- RRV infects NOD mice.
- Monkey, but not human, rotaviruses replicate to a high titer in NOD mouse islets.
- RRV replicates to higher titers in NOD mouse islets than in NOR mouse islets.
- Monkey, but not human, rotaviruses replicate in porcine islets.
- Human and monkey rotaviruses replicate in monkey islets.
- Relative growth of rotaviruses in islets of different species.
- ACKNOWLEDGMENTS
- FOOTNOTES
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