Article Figures & Data
Figures
- FIG. 1.
Location of L domains in retroviral Gag proteins and in the matrix proteins of the rhabdoviruses and filoviruses. The retroviral Gag proteins that harbor L domains are shown in red; matrix, capsid, and nucleocapsid proteins are depicted in green, blue, and yellow, respectively. L domain motifs (PTAP, PPXY, and YXXL) are indicated.
- FIG. 2.
EM analysis of HIV-1 L domain defects. (A) Effect of HIV-1 L domain mutation on virus budding. Virus expression was directed by WT pNL4-3 and a p6 mutant derivative truncated at p6 amino acid 1 (L1Term [36]). Budding from HeLa cells and the Jurkat T-cell line is shown. Adapted from reference 13. (B) Effect of TSG-5′ overexpression on HIV-1 budding. HeLa cells were transfected with pNL4-3 alone (WT) or cotransfected with pNL4-3 plus TSG-5′ (WT + TSG-5′). Adapted with permission from reference 12. In panels A and B, closed arrows indicate virus particles tethered to the plasma membrane; open arrows highlight virion-virion tethers. Bar, 100 nm.
- FIG. 3.
Schematic organization of TSG101 and Nedd4. Domains (described in the text) are indicated. Diagram is not intended to be to scale.
