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Structure and Assembly

Herpes Simplex Virus Nucleocapsids Mature to Progeny Virions by an Envelopment → Deenvelopment → Reenvelopment Pathway

J. N. Skepper, A. Whiteley, H. Browne, A. Minson
J. N. Skepper
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A. Whiteley
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H. Browne
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A. Minson
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DOI: 10.1128/JVI.75.12.5697-5702.2001
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    Fig. 1.

    Immunogold labeling of cells infected with HSV-1 expressing wild-type gD. (A) Decoration of inner and outer nuclear membranes and particles in the perinuclear space; (B and C) decoration of extracellular particles. Bars, 100 nm.

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    Fig. 2.

    Immunogold labeling of cells infected with HSV-1 expressing an ER-targeted gD. (A) Decoration of particles in the perinuclear space; (B and E) decoration of inner and outer nuclear membranes; (D) enlargement of the particle shown in micrograph A that appears to be budding through the inner nuclear membrane; (C and F) extracellular particles. The arrows in micrographs B, D, and E indicate unenveloped capsids in the nucleus. The arrow in micrograph F indicates a very rare example of a decorated extracellular particle. Bars, 100 nm.

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  • Table 1.

    Frequency of gold labeling in perinuclear and extracellular compartments

    Expt no.VirusLocationNo. of enveloped virionsNo. of gold particlesNo. of gold particles/virion
    1Wild typePerinuclear42721.7
    Extracellular1162462.1
    SC16gD-KKXXPerinuclear21683.2
    Extracellular70180.26
    2SC16gD-KKXXPerinuclear9323.6
    Extracellular143150.10
  • Table 2.

    Labeling density of membranes in wild-type-infected cells

    MembraneNo. of gold particles (mean ± SD)/μm of membrane ina:
    gD antibodyControl antibody
    Nuclear5.9 ± 0.50.11 ± 0.02
    Cytoplasmic15.2 ± 3.10.15 ± 0.04
    Plasma0.26 ± 0.030.07 ± 0.01
    • a The number of gold particles per micrometer of membrane were calculated for individual cell profiles. The mean and standard error are given for 10 profiles (using gD antibody) or 8 profiles (control antibody).

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Herpes Simplex Virus Nucleocapsids Mature to Progeny Virions by an Envelopment → Deenvelopment → Reenvelopment Pathway
J. N. Skepper, A. Whiteley, H. Browne, A. Minson
Journal of Virology Jun 2001, 75 (12) 5697-5702; DOI: 10.1128/JVI.75.12.5697-5702.2001

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Herpes Simplex Virus Nucleocapsids Mature to Progeny Virions by an Envelopment → Deenvelopment → Reenvelopment Pathway
J. N. Skepper, A. Whiteley, H. Browne, A. Minson
Journal of Virology Jun 2001, 75 (12) 5697-5702; DOI: 10.1128/JVI.75.12.5697-5702.2001
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