Article Figures & Data
Figures
- Fig. 1.
Immunogold labeling of cells infected with HSV-1 expressing wild-type gD. (A) Decoration of inner and outer nuclear membranes and particles in the perinuclear space; (B and C) decoration of extracellular particles. Bars, 100 nm.
- Fig. 2.
Immunogold labeling of cells infected with HSV-1 expressing an ER-targeted gD. (A) Decoration of particles in the perinuclear space; (B and E) decoration of inner and outer nuclear membranes; (D) enlargement of the particle shown in micrograph A that appears to be budding through the inner nuclear membrane; (C and F) extracellular particles. The arrows in micrographs B, D, and E indicate unenveloped capsids in the nucleus. The arrow in micrograph F indicates a very rare example of a decorated extracellular particle. Bars, 100 nm.
Tables
- Table 1.
Frequency of gold labeling in perinuclear and extracellular compartments
Expt no. Virus Location No. of enveloped virions No. of gold particles No. of gold particles/virion 1 Wild type Perinuclear 42 72 1.7 Extracellular 116 246 2.1 SC16gD-KKXX Perinuclear 21 68 3.2 Extracellular 70 18 0.26 2 SC16gD-KKXX Perinuclear 9 32 3.6 Extracellular 143 15 0.10 - Table 2.
Labeling density of membranes in wild-type-infected cells
Membrane No. of gold particles (mean ± SD)/μm of membrane ina: gD antibody Control antibody Nuclear 5.9 ± 0.5 0.11 ± 0.02 Cytoplasmic 15.2 ± 3.1 0.15 ± 0.04 Plasma 0.26 ± 0.03 0.07 ± 0.01 a The number of gold particles per micrometer of membrane were calculated for individual cell profiles. The mean and standard error are given for 10 profiles (using gD antibody) or 8 profiles (control antibody).
