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Structure and Assembly

Molecular Characterization of a Bacteriophage (Chp2) from Chlamydia psittaci

B. L. Liu, J. S. Everson, B. Fane, P. Giannikopoulou, E. Vretou, P. R. Lambden, I. N. Clarke
B. L. Liu
Department of Molecular Microbiology, University Medical School, Southampton General Hospital, Southampton SO16 6YD, United Kingdom;
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J. S. Everson
Department of Molecular Microbiology, University Medical School, Southampton General Hospital, Southampton SO16 6YD, United Kingdom;
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B. Fane
Department of Veterinary Science and Microbiology, The University of Arizona, Tucson, Arizona 85721-0090; and
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P. Giannikopoulou
Biotechnology, Hellenic Pasteur Institute, 115 21 Athens, Greece
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E. Vretou
Biotechnology, Hellenic Pasteur Institute, 115 21 Athens, Greece
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P. R. Lambden
Department of Molecular Microbiology, University Medical School, Southampton General Hospital, Southampton SO16 6YD, United Kingdom;
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I. N. Clarke
Department of Molecular Microbiology, University Medical School, Southampton General Hospital, Southampton SO16 6YD, United Kingdom;
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DOI: 10.1128/JVI.74.8.3464-3469.2000
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ABSTRACT

Comparisons of the proteome of abortifacient Chlamydia psittaci isolates from sheep by two-dimensional gel electrophoresis identified a novel abundant protein with a molecular mass of 61.4 kDa and an isoelectric point of 6.41. C-terminal sequence analysis of this protein yielded a short peptide sequence that had an identical match to the viral coat protein (VP1) of the avian chlamydiaphage Chp1. Electron microscope studies revealed the presence of a 25-nm-diameter bacteriophage (Chp2) with no apparent spike structures. Thin sections of chlamydia-infected cells showed that Chp2 particles were located to membranous structures surrounding reticulate bodies (RBs), suggesting that Chp2 is cytopathic for ovine C. psittaci RBs. Chp2 double-stranded circular replicative-form DNA was purified and used as a template for DNA sequence analysis. The Chp2 genome is 4,567 bp and encodes up to eight open reading frames (ORFs); it is similar in overall organization to the Chp1 genome. Seven of the ORFs (1 to 5, 7, and 8) have sequence homologies with Chp1. However, ORF 6 has a different spatial location and no cognate partner within the Chp1 genome. Chlamydiaphages have three viral structural proteins, VP1, VP2, and VP3, encoded by ORFs 1 to 3, respectively. Amino acid residues in the φX174 procapsid known to mediate interactions between the viral coat protein and internal scaffolding proteins are conserved in the Chp2 VP1 and VP3 proteins. We suggest that VP3 performs a scaffolding-like function but has evolved into a structural protein.

  • Copyright © 2000 American Society for Microbiology
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Molecular Characterization of a Bacteriophage (Chp2) from Chlamydia psittaci
B. L. Liu, J. S. Everson, B. Fane, P. Giannikopoulou, E. Vretou, P. R. Lambden, I. N. Clarke
Journal of Virology Apr 2000, 74 (8) 3464-3469; DOI: 10.1128/JVI.74.8.3464-3469.2000

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Molecular Characterization of a Bacteriophage (Chp2) from Chlamydia psittaci
B. L. Liu, J. S. Everson, B. Fane, P. Giannikopoulou, E. Vretou, P. R. Lambden, I. N. Clarke
Journal of Virology Apr 2000, 74 (8) 3464-3469; DOI: 10.1128/JVI.74.8.3464-3469.2000
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KEYWORDS

bacteriophages
Chlamydophila psittaci

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