Article Figures & Data
Figures
- Fig. 1.
Time course of SIVsmH-4-neutralizing antibody production following intravenous inoculation of vaccinated macaques with SIVsmE660. SIVsmH-4-neutralizing antibodies were measured in plasma samples obtained on the day of challenge and at multiple time points for 12 weeks postchallenge. Titers of neutralizing antibodies are the reciprocal plasma dilution at which 50% of CEMx174 cells were protected from virus-induced cell killing. Undetectable neutralization was given a value of 30, which was the lowest reciprocal plasma dilution tested. Panel A, MVA-gag-pol; panel B, MVA-env; panel C, MVA-gag-pol-env; panel D, MVA. †, animals sacrificed because of clinical manifestations of AIDS; ✞, animal that died of causes unrelated to AIDS.
- Fig. 2.
Time course of challenge-strain-neutralizing antibody production following intravenous inoculation of vaccinated macaques with SIVsmE660. SIVsmE660-neutralizing antibodies were measured in plasma samples obtained on the day of challenge and at multiple time points for 28 weeks postchallenge. Titers of neutralizing antibodies are the reciprocal plasma dilution at which 50% of CEMx174 cells were protected from virus-induced cell killing. Undetectable neutralization was given a value of 30, which was the lowest reciprocal plasma dilution tested. Panel A, MVA-gag-pol; panel B, MVA-env; panel C, MVA-gag-pol-env; panel D, MVA. †, animals sacrificed because of clinical manifestations of AIDS; ✞, animal that died of causes unrelated to AIDS.
Tables
- Table 1.
Magnitude and cross-reactivity of neutralizing antibodies detected 2 weeks post-virus challenge
Vaccine group and animal no.a Nab titer to SIV ofb: smH-4 DeltaB670 mac251 MVA-SIV-gag-pol A4 <30 <20 <20 A5 420 31 <20 A6 396 30 <20 MVA-SIV-env B1 28,478 2,116 45 B5 43,680 5,947 831 B6 60,309 3,483 37 MVA-SIV-gag-pol-env C1 50,072 6,034 969 C5 42,297 3,700 958 C6 48,938 2,561 48 MVA D1 <20 <20 <20 D2 <20 <20 <20 D4 <20 <20 44 ↵a Plasma samples from three animals in each of four immunization groups were obtained 2 weeks post-intravenous challenge with SIVsmE660.
↵b Plasma samples were assessed for antibodies neutralizing SIVsmH-4, SIV/DeltaB670, and SIVmac251 in CEMx174 cells as described in the text. Titers of neutralizing antibodies are the reciprocal plasma dilution at which 50% of cells were protected from virus-induced killing.
- Table 2.
Magnitude and cross-reactivity of neutralizing antibodies detected 8 weeks post-virus challenge
Vaccine group and animal no.a Nab titer to SIV ofb: smH-4 E660 DeltaB670 mac251 mac239 MVA-SIV-gag-pol A4 5,788 <30 2,011 476 <20 A5 5,539 <30 1,978 343 <20 A6 14,168 <30 3,713 503 <20 MVA-SIV-env B1 26,347 <30 1,968 162 <20 B5 11,279 <30 1,599 756 <20 B6 11,546 <30 506 <20 <20 MVA-SIV-gag-pol-env C1 29,281 <30 4,127 858 <20 C5 16,310 180 2,950 1,015 <20 C6 19,422 <30 2,494 <20 <20 MVA D1 10,633 <30 4,689 661 <20 D2 3,206 <30 1,118 345 <20 D4 7,405 <30 2,465 337 <20 ↵a Plasma samples from three animals in each of four immunization groups were obtained 8 weeks post-intravenous challenge with SIVsmE660.
↵b Plasma samples were assessed for antibodies neutralizing SIVsmH-4, SIVsmE660, SIV/DeltaB670, SIVmac251, and molecularly cloned SIVmac239 in CEMx174 cells as described in the text. Titers of neutralizing antibodies are the reciprocal plasma dilution at which 50% of cells were protected from virus-induced killing.
- Table 3.
Comparative neutralization of the assay and animal challenge stocks of SIVsmE660
Animal no.a Nab titer measured with SIVsmE660b: Cell viability Reduction in p27 Assay stock Challenge stock Challenge stock B5 1,082 1,340 2,118 B6 1,841 7,502 7,405 C1 470 384 181 ↵a Plasma samples were obtained 28 weeks post-intravenous inoculation with SIVsmE660.
↵b Neutralization was measured with the assay stock of SIVsmE660 grown in CEMx174 cells and the animal challenge stock of the same virus grown in macaque PBMC. Assays were performed with CEMx174 cells as described in the text with one modification: cells exposed to the animal challenge stock of virus were washed three times with growth medium 1 day after the addition of cells in order to remove the virus inoculum and residual anti-p27 antibodies. Culture supernatants were harvested for p27 quantification at the time cells were stained for viability. Neutralization titers are the reciprocal plasma dilution where either 50% of cells were protected from virus-induced killing or p27 synthesis was reduced 90% relative to virus control wells (no plasma sample). The average amount of p27 present in the virus control wells was 95 ng/ml.
