Article Figures & Data
Figures
- Fig. 1.
RV M protein is positioned between the lipid bilayer and the RNP coil. Virions treated with Triton X-100 (B to D) and untreated control samples (A) were purified as described in Materials and Methods and analyzed by electron microscopy. The absence of anti-M labeling on the outer membranes of untreated virions (90 to 100 nm in diameter) (A) and an intense anti-M labeling on the surfaces of the RNP coil or skeletons (50 to 60 nm in diameter) of detergent-treated virions (C) demonstrate that M surrounds the RNP coil. Only areas where the lipid bilayer was not completely removed (60 to 70 nm in diameter) are weakly labeled with anti-G MAb (B). In partially disassembled skeletons (D), only the uncoiled nucleocapsid, and not the surface of the skeleton, is labeled with anti-RNP serum. Bar, 100 nm.
- Fig. 2.
Organization of the M-deficient RV genome. The entire RV genome with its five open reading frames (top), a detailed M cistron region (middle), and the region encompassing a deletion (bottom) are shown. Arrowheads and bars represent transcriptional start and stop signals, respectively. In SAD ΔM, the entire M cistron (SAD L16 nucleotides 2435 to 3176) was removed by a deletion starting within the nontranslated region of the P gene and ending close to the transcriptional stop signal of the M gene.
- Fig. 3.
SAD ΔM induces increased cell-cell fusion and cell death. (A) Following infection of cultures with an MOI of 0.005, live cells were examined microscopically for morphological changes at the indicated times. Cell fusion is detected in SAD ΔM-infected cells after 48 h, and large syncytia are present at 72 h postinfection. (B) In cells infected in parallel, the spread of infection was monitored at the indicated times by direct immunofluorescence with a conjugate directed against RV N protein. Secondary infection of cells, as indicated by the appearance of fluorescing granules, is virtually absent in SAD ΔM. After 48 h, SAD ΔM N expression is found in large syncytia.
- Fig. 4.
Analysis of cell surface expression of G proteins. Approximately 106 BSR cells were infected at an MOI of 1 and after 16 h were labeled with 100 μCi of [35S]methionine for 3 h. Anti-G MAb was incubated with live cells to bind G proteins expressed at the cell surface or with cell lysates containing total G protein. Phoshorimager quantitation revealed a 2.6-fold-higher level of G protein on the surfaces of cells infected by SAD ΔM.
- Fig. 5.
Protein composition of cell-associated SAD L16 and SAD ΔM virions. Cell extracts from approximately 106infected cells were prepared as described in Materials and Methods and purified by 10 to 70% sucrose gradient centrifugation. Twelve equal gradient fractions (numbered from top to bottom) were analyzed by Western blotting for the presence of viral proteins. The sucrose density was determined from the refractive index of each fraction. For both preparations, the peak of infectivity was present in fraction 6, which has a density of 1.14 g/cm3.
- Fig. 6.
RV M interacts with G. Approximately 106 BSR cells were first infected with vTF7-3 and then transfected with 5 μg of plasmid DNA. After 16 h of infection, cell lysates were prepared, centrifuged to equilibrium, and analyzed as described for Fig. 5. (A) Density gradients from cells expressing G, N, or M protein individually; (B) cells coexpressing G and N proteins or G and M proteins. When G and M proteins were coexpressed, the G protein cosedimented with M protein (numbering is from the top to the bottom of the gradient).
- Fig. 7.
Morphology of particles released from SAD ΔM-infected cells. SAD ΔM was grown in the absence of RV M protein (A to C) or in cells that express RV M protein from transfected plasmids (D). Purified supernatants from approximately 107 SAD ΔM-infected BSR cells were immunostained with a MAb specific for the RV G protein and analyzed by electron microscopy. In the absence of RV M protein, either long rod-shaped particles (A and B) or round vesicular structures (C), but no bullet-shaped particles, were detected. After expression of RV M protein, the majority of particles had the typical bullet-shaped rhabdovirus morphology (D). Bar, 100 nm.
Tables
- Table 1.
Titers of released and cell-associated RV
Virus h post-infection Focus-forming units/mla in: % Cell-associated virusb Supernatant Cell lysate SAD L16 24 3.5 × 106 4.5 × 106 56 SAD ΔM 24 6 × 101 1.2 × 104 99.5 SAD L16 48 1.6 × 108 1.5 × 107 8.6 SAD ΔM 48 3 × 102 1.5 × 104 98 SAD L16 72 8 × 107 1 × 106 1.3 SAD ΔM 72 1.8 × 102 7.5 × 103 97.7
