Article Figures & Data
Figures
- Fig. 1.
RFLP mapping of the cloned receptor gene. Genomic DNA from the parental lines, 72 and 63, and 18 F2 progeny was digested with TaqI and then analyzed by Southern blotting with a random-primer-labeled 375-bp probe specific for the cloned ASLV-A receptor gene. The approximate sizes (in kilobases) of the two hybridizing fragments are indicated. The susceptibility of the F2 progeny to ASLV-A infection is indicated. R, resistant; S, sensitive.
- Fig. 2.
Sequence analysis of the viral interaction region in the ALSV-A receptor. (A) The viral interaction region from the ASLV-A receptor gene in lines 63 and 72 was amplified by PCR and the DNA sequence was determined. The deduced amino acid sequence and DNA sequence for line 63 are shown in the top lines. Nucleotide differences between the two chicken lines are indicated by capital letters, and dots represent identical sequences. For line 72, only the altered amino acid residues are shown. The polymorphic TaqI site is underlined. (B) The viral interaction region of the receptor gene from genomic DNA of randomly bred broiler-type chickens was amplified by PCR and directly sequenced. The deduced amino acid sequence and susceptibility to ASLV-A infection are shown compared to the line 63 sequence. Dots indicate identical residues. R, resistant to ASLV-A; S, sensitive to ASLV-A. C/O represents the sequence of the functional ASLV-A receptor gene identified by gene transfer (19).
Tables
- Table 1.
Segregation of resistance and susceptibility to subgroup A and B viruses in F2 progeny obtained by crossing lines 63 and 72
Subgroup B susceptibility phenotypea No. of progeny with subgroup A susceptibility phenotypeb Total Susceptible Resistant Susceptible 45 22 67 Resistant 17 9 26 Total 62 31 ↵a Susceptibility to subgroup B viruses was determined by injection of 500 FFU of Bryan high-titer RSV (RAV-2) into the wing webs of 4-week-old chicks. Tumors were scored on the basis of palpation of the wing web at 2, 3, and 4 weeks postinfection.
↵b Susceptibility to subgroup A viruses was determined as described for subgroup B except that Bryan high-titer RSV (RAV-1) was used.
