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Virus-Cell Interactions

Genetic Mapping of the Cloned Subgroup A Avian Sarcoma and Leukosis Virus Receptor Gene to the TVALocus

Paul Bates, Lijun Rong, Harold E. Varmus, John A. T. Young, Lyman B. Crittenden
Paul Bates
Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104-6076;
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Lijun Rong
Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104-6076;
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Harold E. Varmus
National Institutes of Health, Bethesda, Maryland 20892;
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John A. T. Young
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115; and
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Lyman B. Crittenden
USDA Avian Disease and Oncology Laboratory and Department of Microbiology, Michigan State University, East Lansing, Michigan 48824
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DOI: 10.1128/JVI.72.3.2505-2508.1998
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    Fig. 1.

    RFLP mapping of the cloned receptor gene. Genomic DNA from the parental lines, 72 and 63, and 18 F2 progeny was digested with TaqI and then analyzed by Southern blotting with a random-primer-labeled 375-bp probe specific for the cloned ASLV-A receptor gene. The approximate sizes (in kilobases) of the two hybridizing fragments are indicated. The susceptibility of the F2 progeny to ASLV-A infection is indicated. R, resistant; S, sensitive.

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    Fig. 2.

    Sequence analysis of the viral interaction region in the ALSV-A receptor. (A) The viral interaction region from the ASLV-A receptor gene in lines 63 and 72 was amplified by PCR and the DNA sequence was determined. The deduced amino acid sequence and DNA sequence for line 63 are shown in the top lines. Nucleotide differences between the two chicken lines are indicated by capital letters, and dots represent identical sequences. For line 72, only the altered amino acid residues are shown. The polymorphic TaqI site is underlined. (B) The viral interaction region of the receptor gene from genomic DNA of randomly bred broiler-type chickens was amplified by PCR and directly sequenced. The deduced amino acid sequence and susceptibility to ASLV-A infection are shown compared to the line 63 sequence. Dots indicate identical residues. R, resistant to ASLV-A; S, sensitive to ASLV-A. C/O represents the sequence of the functional ASLV-A receptor gene identified by gene transfer (19).

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  • Table 1.

    Segregation of resistance and susceptibility to subgroup A and B viruses in F2 progeny obtained by crossing lines 63 and 72

    Subgroup B susceptibility phenotypeaNo. of progeny with subgroup A susceptibility phenotypebTotal
    SusceptibleResistant
    Susceptible452267
    Resistant17926
    Total6231
    • ↵a Susceptibility to subgroup B viruses was determined by injection of 500 FFU of Bryan high-titer RSV (RAV-2) into the wing webs of 4-week-old chicks. Tumors were scored on the basis of palpation of the wing web at 2, 3, and 4 weeks postinfection.

    • ↵b Susceptibility to subgroup A viruses was determined as described for subgroup B except that Bryan high-titer RSV (RAV-1) was used.

  • Table 2.

    Cosegregation of the TaqI RFLP and TVA phenotype

    TVA phenotypeaSize(s) (kb) of TaqI fragment(s) observedbNo. of F2progeny
    Susceptible2.416
    Susceptible2.4, 3.046
    Resistant3.031
    • ↵a The TVA phenotype was scored by wing web injection of Bryan high-titer RSV (RAV-1) as described in TABLE 1, footnote a.

    • ↵b Sizes of fragments observed after hybridization with the exon 3 probe from the cloned ASLV-A receptor gene.

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Genetic Mapping of the Cloned Subgroup A Avian Sarcoma and Leukosis Virus Receptor Gene to the TVALocus
Paul Bates, Lijun Rong, Harold E. Varmus, John A. T. Young, Lyman B. Crittenden
Journal of Virology Mar 1998, 72 (3) 2505-2508; DOI: 10.1128/JVI.72.3.2505-2508.1998

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Genetic Mapping of the Cloned Subgroup A Avian Sarcoma and Leukosis Virus Receptor Gene to the TVALocus
Paul Bates, Lijun Rong, Harold E. Varmus, John A. T. Young, Lyman B. Crittenden
Journal of Virology Mar 1998, 72 (3) 2505-2508; DOI: 10.1128/JVI.72.3.2505-2508.1998
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KEYWORDS

avian leukosis virus
Avian Sarcoma Viruses
Chromosome Mapping
Receptors, Virus

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