Article Figures & Data
Figures
- Fig. 1.
Mean clinical disease scores for conjunctivitis and blepharitis (eyelid skin inflammation) in mice infected with HSV-1 (•) and mock-inoculated mice (○). Eyes were examined at ×3 magnification and with a focal light source before inoculation and at 1, 3, 5, 11, 18, 23, 29, and 37 days p.i. Clinical signs of conjunctivitis (A) and blepharitis (B) were scored and the MDS were calculated for each group of mice on each day of observation.
- Fig. 2.
In situ PCR and immunoperoxidase labeling for HSV-1 and histological lesions in sections of acutely infected conjunctivae. (A) In situ PCR labeling of HSV-1 DNA in conjunctival cells at 5 days p.i. with HSV-1. The sections shown in panels B to D were in the same series and obtained from sites near the section shown in panel A. (B) Hematoxylin- and eosin-stained section. Note the inflammatory cell infiltration of the epithelium and substantia propria and the necrosis of the epithelium. (C) In situ PCR testing with primers for a feline herpesvirus type 1 gene. Note the absence of labeling. (D) Immunoperoxidase labeling of HSV-1 antigen. (E) In situ PCR testing of a section of conjunctiva from a mock-inoculated mouse at 5 days p.i. Note the absence of labeling. Bar, 19.6 μm.
- Fig. 3.
In situ PCR labeling of HSV-1 DNA and histological lesions in sections of chronically infected conjunctiva of the third eyelid. (A) In situ PCR labeling of HSV-1 DNA in conjunctival cells at 23 days p.i. with HSV-1. Inset shows, at higher magnification, the epithelial cell marked by an arrow in the main photomicrograph. (B) Hematoxylin- and eosin-stained section in the same series obtained from a site near the section shown in panel A. Note the inflammatory cell infiltration of the epithelium and substantia propria. Inset shows, at higher magnification, the focus of neutrophils and macrophages indicated by an arrow in the main photomicrograph. (C) In situ PCR testing of a section of the conjunctiva of the third eyelid from a mock-inoculated mouse at 11 days p.i. Note the absence of labeling. Bar, 19.6 μm in the main photomicrographs and 11.5 μm in the insets.
- Fig. 4.
In situ PCR labeling of HSV-1 DNA in sections of chronically infected eyelid skin. (A) In situ PCR labeling of HSV-1 DNA in follicular epithelial cells at 37 days p.i. with HSV-1. The epithelial cell indicated by an arrow is shown at higher magnification in the inset. (B) Hematoxylin- and eosin-stained section in the same series obtained from near the section shown in panel A. Note the inflammatory cell infiltration of follicular epithelium and surrounding dermis. (C) In situ PCR testing of a section of eyelid skin including both epidermal and follicular epithelial cells from a mock-inoculated mouse at 11 days p.i.; note the absence of labeling. (D) In situ PCR labeling of HSV-1 DNA in epidermal cells of eyelid skin at 37 days p.i. with HSV-1. (E) Hematoxylin- and eosin-stained section in the same series obtained from near the section shown in panel D. Note the inflammatory cell infiltration in the epidermis and dermis. Bar, 19.6 μm in the main photomicrographs and 11.5 μm in the inset.
- Fig. 5.
Percentages of eyes in which viral DNA (░), histological lesions (■), and viral antigen (□) could be detected in the conjunctiva (A) and eyelid skin (B) at different times p.i. with HSV-1. All groups included 10 eyes.
