Article Figures & Data
Tables
- Table 1.
RSV-specific CTLp frequencies for MHC class I- and II-restricted lymphocytes isolated from BAL fluid of BALB/c mice at various time points post-i.n. infection with RSV or an HPIV3 controla
No. of days p.i.b Virus MHC class CTLp frequency rangec 3 RSV I >106 3 RSV II 106 5 RSV I 1:46,500–1:50,200 5 RSV II 1:145,000–1:162,100 7 RSV I 1:5,300–1:6,500 7 RSV II 1:22,800–1:28,500 7 HPIV3 I >106 10 RSV I 1:2,800–1:6,300 10 RSV II 1:25,500–1:32,200 10 HPIV3 I >106 14 RSV I 1:2,500–1:7,500 14 RSV II 1:12,500–1:38,000 48 RSV I 1:120,800–1:230,000 48 RSV II 1:210,500–1:>106 a Three individual mice were examined at each time point for the frequencies of class I-and II-restricted CTLps, and the range of frequencies is shown.
b Number of days after RSV infection.
c The 95% confidence limits for all of the CTLp frequencies shown ranged from 1,200 to 26,300. The limiting-dilution protocol used 51Cr-labeled, RSV-infected target cells and uninfected target cells to determine the background. The values for uninfected targets were always >1:500,000 and are not shown. The CTLp frequency for naive animals was >1:106.
- Table 2.
RSV-specific CTLp frequencies for MHC class I- and II-restricted lymphocytes isolated from the spleens of BALB/c mice at various time points post-i.n. infection with RSV or an HPIV3 controla
No. of days p.i.b Virus MHC class CTLp frequency rangec 3 RSV I >106 3 RSV II >106 4 RSV I 1:29,200–1:37,900 4 RSV II 1:71,800–1:75,700 5 RSV I 1:2,200–1:8,300 5 RSV II 1:2,300–1:12,100 7 RSV I 1:4,800–1:6,000 7 RSV II 1:5,500–1:6,300 7 HPIV3 I >106 10 RSV I 1:5,000–1:6,300 10 RSV II 1:2,000–1:2,900 10 HPIV3 I >106 12 RSV I 1:4,000–1:6,400 12 RSV II 1:2,200–1:3,300 48 RSV I 1:26,900–1:33,400 48 RSV II 1:14,200–1:21,200 a Three individual mice were examined at each time point for the frequencies of class I-and II-restricted CTLps, and the range of frequencies is shown.
b Number of days after RSV infection.
c The 95% confidence limits for all of the CTLp frequencies shown ranged from 710 to 1,800. The limiting-dilution protocol used 51Cr-labeled, RSV-infected target cells and uninfected target cells to determine the background. The values for uninfected targets were always >1:500,000 and are not shown. The CTLp frequency for naive animals was >1:106.
- Table 3.
RSV-specific CTLp frequencies for MHC class I- and II-restricted lymphocytes isolated from the spleens of BALB/c mice at various time points postvaccination with FI-RSV or an FI-HPIV3 controla
No. of days p.i.b Ag MHC class CTLp frequency rangec 3 FI-RSV I >106 3 FI-RSV II >106 4 FI-RSV I 1:147,000–1:365,300 4 FI-RSV II 1:21,600–1:43,400 5 FI-RSV I 1:80,500–1:183,000 5 FI-RSV II 1:8,800–1:10,900 7 FI-RSV I 1:28,300–1:55,600 7 FI-RSV II 1:2,000–1:3,800 7 FI-HPIV3 I >106 10 FI-RSV I 1:14,600–1:18,700 10 FI-RSV II 1:1,700–1:3,300 10 FI-HPIV3 I >106 12 FI-RSV I 1:15,800–1:31,100 12 FI-RSV II 1:1,500–1:2,600 48 FI-RSV I 1:38,500–1:65,200 48 FI-RSV II 1:2,700–1:3,900 a Three individual mice were examined at each time point for the frequencies of class I-and II-restricted CTLps, and the ranges are shown.
b Number of days after immunization with FI-RSV.
c The 95% confidence limits for class I-restricted CTLps ranged from 1,000 to 9,000. The 95% confidence limits for class II-restricted CTLPs ranged from 720 to 1,300. The limiting-dilution protocol used 51Cr-labeled, RSV-infected target cells and uninfected target cells to determine the background. The values for uninfected targets were always >1:500,000 and are not shown.
- Table 4.
RSV-specific CTLp frequencies for MHC class I- and II-restricted lymphocytes isolated from the spleens of BALB/c mice challenged with RSV or HPIV3 3 weeks after intramuscular immunization with FI-RSV or FI-HPIV3a
No. of days p.i.b Virus MHC class CTLp frequency rangec Primary Secondary 0 FI-RSV I 1:111,300–1:113,600 0 FI-RSV II 1:11,900–1:12,200 3 FI-RSV RSV I 1:12,600–1:14,400 3 FI-RSV RSV II 1:19,700–1:24,600 4 FI-RSV RSV I 1:2,300–1:3,900 4 FI-RSV RSV II 1:8,200–1:12,700 6 FI-RSV RSV I 1:980–1:5,700 6 FI-RSV RSV II 1:3,100–1:8,200 3 RSV I >106 3 RSV II >106 6 RSV I 1:4,800–1:6,000 6 RSV II 1:5,800–1:6,500 0 FI-HPIV3 I >106 3 FI-HPIV3 RSV I >106 5 FI-HPIV3 RSV I 1:14,200–1:24,900d 3 HPIV3 I >106 5 HPIV3 I >106 3 FI-HPIV3 HPIV3 I 1:856,300–>106 5 FI-HPIV3 HPIV3 I >106 a Three individual mice were examined at each time point for the frequencies of class I-and II-restricted CTLps, and the frequency ranges are shown.
b Number of days after RSV challenge.
c The 95% confidence limits for the CTLp frequencies shown ranged from 680 to 1,800. The limiting-dilution protocol used 51Cr-labeled, RSV-infected target cells and uninfected target cells to determine the background. The values for uninfected targets were always >1:500,000 and are not shown. The RSV-specific CTLp frequencies for age-matched control mice infected with RSV alone are shown.
d The 95% confidence limits for the CTLp frequencies shown ranged from 500 to 720.
