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Virus-Cell Interactions

Cellular Localization of Latent Murine Cytomegalovirus

Alan J. Koffron, Mary Hummel, Bruce K. Patterson, Shixian Yan, Dixon B. Kaufman, Jonathan P. Fryer, Frank P. Stuart, Michael I. Abecassis
Alan J. Koffron
Department of Surgery, Division of Organ Transplantation,
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Mary Hummel
Department of Surgery, Division of Organ Transplantation,
Department of Microbiology and Immunology, and
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Bruce K. Patterson
Departments of Obstetrics & Gynecology and Medicine, Northwestern University Medical School, Chicago, Illinois 60611
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Shixian Yan
Department of Surgery, Division of Organ Transplantation,
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Dixon B. Kaufman
Department of Surgery, Division of Organ Transplantation,
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Jonathan P. Fryer
Department of Surgery, Division of Organ Transplantation,
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Frank P. Stuart
Department of Surgery, Division of Organ Transplantation,
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Michael I. Abecassis
Department of Surgery, Division of Organ Transplantation,
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DOI: 10.1128/JVI.72.1.95-103.1998
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ABSTRACT

Herpesviruses typically establish latent infection in their hosts. The cell(s) responsible for harboring latent virus, in most cases, is not known. Using immunofluorescence and PCR-in situ hybridization (PISH), a technique which combines the sensitivity of PCR with the localization and specificity of in situ hybridization, we provide the first direct evidence that endothelial cells are a major site of murine cytomegalovirus (MCMV) DNA in latently infected animals. These findings are consistent with existing knowledge of the biological behavior of CMV, in particular the transmission of latent CMV by solid organ and bone marrow transplantation, in both human and animal models. In addition, we have localized MCMV DNA in the lung alveolar macrophage and in bone marrow cells. Our findings confirm that bone marrow-derived hematopoietic cells are a site of CMV latency and further suggest that bone marrow may be a reservoir of infected progeny capable of migrating into the circulation and establishing latency in various tissues. These findings provide clearly needed insight into the site of latent infection which is central to an understanding of the mechanisms of reactivation.

  • Copyright © 1998 American Society for Microbiology
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Cellular Localization of Latent Murine Cytomegalovirus
Alan J. Koffron, Mary Hummel, Bruce K. Patterson, Shixian Yan, Dixon B. Kaufman, Jonathan P. Fryer, Frank P. Stuart, Michael I. Abecassis
Journal of Virology Jan 1998, 72 (1) 95-103; DOI: 10.1128/JVI.72.1.95-103.1998

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Cellular Localization of Latent Murine Cytomegalovirus
Alan J. Koffron, Mary Hummel, Bruce K. Patterson, Shixian Yan, Dixon B. Kaufman, Jonathan P. Fryer, Frank P. Stuart, Michael I. Abecassis
Journal of Virology Jan 1998, 72 (1) 95-103; DOI: 10.1128/JVI.72.1.95-103.1998
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KEYWORDS

Herpesviridae Infections
Muromegalovirus

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