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Journal Article | Research Support, U.S. Gov't, P.H.S.

Expression of human immunodeficiency virus type 1 reverse transcriptase in trans during virion release and after infection.

M A Ansari-Lari, R A Gibbs
M A Ansari-Lari
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R A Gibbs
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ABSTRACT

The normal reverse transcription of retroviral RNA is a complex process which depends on the orchestration of several steps throughout the virus life cycle. During the assembly of retroviruses, reverse transcriptase (RT) is directed into the virion as a component of the Gag-Pol polyprotein. In the maturation of the Gag-Pol polyprotein of human immunodeficiency virus type 1 (HIV-1), cleavage by the viral protease occurs during viral budding. After infection, reverse transcription of viral RNA into double-stranded DNA is completed in the cytoplasm of the infected cell. In this study, the processing and reverse transcription of HIV-1 have been examined by separate expression of mature HIV-1 RT and proviral molecules bearing RT mutations. The effects of RT expression in trans during virion release and after viral entry were investigated. Constitutive expression of HIV-1 RT was established in CD4- and non-CD4-expressing cells via the coexpression of its individual subunits, and three HIV-1 RT mutant constructs were generated. The results indicate that a bona fide RT trans complementation does not occur during virion release or after infection. However, after infection of an RT-expressing cell with a high titer RT-defective virus, intracellular reverse transcription can be detected.

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Expression of human immunodeficiency virus type 1 reverse transcriptase in trans during virion release and after infection.
M A Ansari-Lari, R A Gibbs
Journal of Virology Jun 1996, 70 (6) 3870-3875; DOI:

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Expression of human immunodeficiency virus type 1 reverse transcriptase in trans during virion release and after infection.
M A Ansari-Lari, R A Gibbs
Journal of Virology Jun 1996, 70 (6) 3870-3875; DOI:
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