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Journal Article | Research Support, Non-U.S. Gov't | Research Support, U.S. Gov't, P.H.S.

Mature B cells are required for acute splenic infection, but not for establishment of latency, by murine gammaherpesvirus 68.

K E Weck, M L Barkon, L I Yoo, S H Speck, I V Virgin HW
K E Weck
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M L Barkon
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L I Yoo
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S H Speck
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I V Virgin HW
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ABSTRACT

Murine gammaherpesvirus 68 (gamma HV-68; also referred to as MHV-68) is a gammaherpesvirus which infects murid rodents. Previous studies showed that CD8 T cells are important for controlling gamma HV-68 replication during the first 2 weeks of infection and suggested a role for B cells in latent or persistent gamma HV-68 infection. To further define the importance of B cells and CD8 T cells during acute and chronic gamma HV-68 infection, we examined splenic infection in mice with null mutations in the transmembrane domain of the mu-heavy-chain constant region (MuMT; B-cell and antibody deficient) or in the beta2-microglobulin gene (beta2 -/-; CD8 deficient). Immunocompetent mice infected intraperitoneally with gamma HV-68 demonstrated peak splenic titers 9 to 10 days postinfection, cleared infectious virus 15 to 20 days postinfection, and harbored low levels of latent virus at 6 weeks postinfection. Beta2-/- mice showed peak splenic gamma HV-68 titers similar to those of normal mice but were unable to clear infectious virus completely from the spleen, demonstrating persistent infectious virus 6 weeks postinfection. These data indicate that CD8 T cells are important for clearing infectious gamma HV-68 from the spleen. Infected MuMT mice did not demonstrate detectable infectious gamma HV-68 in the spleen at any time after infection, indicating that mature B lymphocytes are necessary for acute splenic infection by gamma HV-68. Despite the lack of measurable acute infection, MuMT spleen cells harbored latent virus 6 weeks postinfection at a level about 100-fold higher than that in normal mice. These data demonstrate establishment of latency by a herpesvirus in an organ in the absence of acute viral replication in that organ. In addition, they demonstrate that gamma HV-68 can establish latency in a cell type other than mature B lymphocytes.

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Mature B cells are required for acute splenic infection, but not for establishment of latency, by murine gammaherpesvirus 68.
K E Weck, M L Barkon, L I Yoo, S H Speck, I V Virgin HW
Journal of Virology Oct 1996, 70 (10) 6775-6780; DOI:

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Mature B cells are required for acute splenic infection, but not for establishment of latency, by murine gammaherpesvirus 68.
K E Weck, M L Barkon, L I Yoo, S H Speck, I V Virgin HW
Journal of Virology Oct 1996, 70 (10) 6775-6780; DOI:
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