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Case Reports | Comparative Study | Journal Article | Research Support, Non-U.S. Gov't | Research Support, U.S. Gov't, P.H.S.

Persistence of attenuated rev genes in a human immunodeficiency virus type 1-infected asymptomatic individual.

A K Iversen, E G Shpaer, A G Rodrigo, M S Hirsch, B D Walker, H W Sheppard, T C Merigan, J I Mullins
A K Iversen
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E G Shpaer
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A G Rodrigo
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M S Hirsch
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B D Walker
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H W Sheppard
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T C Merigan
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J I Mullins
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ABSTRACT

With the goal of examining the functional diversity of human immunodeficiency virus type 1 (HIV-1) env genes within the peripheral blood mononuclear cells of an asymptomatic individual, we substituted four complete env genes into the replication-competent NL4-3 provirus. Despite encoding full-length open reading frames for gp120 and gp41 and the second coding exon of tat and rev, each chimera was replication defective. Site-directed mutagenesis of codon 78 in the Rev activation domain (from a hitherto unique Ile to the subtype B consensus Leu) partially restored infectivity for two of three chimeras tested. Similarly, mutagenesis of rev codon 78 of NL4-3 from Leu to Ile partially attenuated this virus. Ile-78 was found in all 13 clones examined from samples taken from this asymptomatic subject 4.5 years after infection, including 9 from peripheral blood mononuclear cells and 4 from a virus isolate, as well as 4 additional clones each from peripheral blood mononuclear cells sampled 37 and 51 months later. We next examined conservation of the Rev activation domain within and among long-term survivors (LTS) and patients with AIDS, as well as T-cell-line-adapted strains of HIV-1. Putative attenuating mutations were found in a minority of sequences from all five LTS and two of four patients with AIDS. Of the 11 T-cell-line-adapted viruses examined, none had these changes. Among and within LTS virus population had marginally higher levels of diversity in Rev than in Env; patients with AIDS had similar levels of diversity in the two reading frames; and T-cell-line-adapted viruses had higher levels of diversity in Env. These results are consistent with the hypothesis that asymptomatic individuals harbor attenuated variants of HIV-1 which correlate with and contribute to their lack of disease progression.

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Persistence of attenuated rev genes in a human immunodeficiency virus type 1-infected asymptomatic individual.
A K Iversen, E G Shpaer, A G Rodrigo, M S Hirsch, B D Walker, H W Sheppard, T C Merigan, J I Mullins
Journal of Virology Sep 1995, 69 (9) 5743-5753; DOI:

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Persistence of attenuated rev genes in a human immunodeficiency virus type 1-infected asymptomatic individual.
A K Iversen, E G Shpaer, A G Rodrigo, M S Hirsch, B D Walker, H W Sheppard, T C Merigan, J I Mullins
Journal of Virology Sep 1995, 69 (9) 5743-5753; DOI:
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