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Research Article

Association of Epstein-Barr virus early antigen diffuse component and virus-specified DNA polymerase activity.

J S Li, B S Zhou, G E Dutschman, S P Grill, R S Tan, Y C Cheng
J S Li
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B S Zhou
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G E Dutschman
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S P Grill
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R S Tan
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Y C Cheng
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ABSTRACT

The role of Epstein-Barr virus (EBV) early antigen diffuse component (EA-D) and its relationship with EBV DNA polymerase in EBV genome-carrying cells are unclear, EBV-specified DNA polymerase was purified in a sequential manner from Raji cells treated with phorbol-12,13-dibutyrate and n-butyrate by phosphocellulose, DEAE-cellulose, double-stranded DNA-cellulose, and blue Sepharose column chromatography. Four polypeptides with molecular masses of 110,000, 100,000, 55,000, and 49,000 daltons were found to be associated with EBV-specified DNA polymerase activity. A monoclonal antibody which could neutralize the EBV DNA polymerase activity was prepared and found to recognize 55,000- and 49,000-dalton polypeptides. An EA-D monoclonal antibody, R3 (G. R. Pearson, V. Vorman, B. Chase, T. Sculley, M. Hummel, and E. Kieff, J. Virol. 47:183-201, 1983), was also able to recognize these same two polypeptides associated with EBV DNA polymerase activity. It was concluded that EBV EA-D polypeptides, as identified by R3 monoclonal antibody, are critical components of EBV DNA polymerase.

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Association of Epstein-Barr virus early antigen diffuse component and virus-specified DNA polymerase activity.
J S Li, B S Zhou, G E Dutschman, S P Grill, R S Tan, Y C Cheng
Journal of Virology Sep 1987, 61 (9) 2947-2949; DOI:

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Association of Epstein-Barr virus early antigen diffuse component and virus-specified DNA polymerase activity.
J S Li, B S Zhou, G E Dutschman, S P Grill, R S Tan, Y C Cheng
Journal of Virology Sep 1987, 61 (9) 2947-2949; DOI:
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