Formation of Cellular Deoxyribonucleic Acid During Productive Polyoma Virus Infection

ABSTRACT

It was previously shown that the majority of deoxyribonucleic acid (DNA) made in growing mouse embryo cells productively infected at low multiplicity with polyoma virus is cellular in nature and that some of this cell DNA contains discontinuities in the newly synthesized strand. Evidence obtained indicates the following. (i) Induction of cell DNA synthesis precedes the onset of detectable viral DNA replication by approximately 3 hr. (ii) Double-stranded cell DNA molecules, discontinuous in the newly synthesized strands, arise by direct synthesis (rather than by degradation of a high-molecular-weight precursor) only in the cell DNA replicated after initiation of viral DNA synthesis. (iii) This DNA component is continuously formed throughout the “late” stage of infection and is continuously converted into apparently normal cell DNA of high molecular weight without prior degradation to acid-soluble components.