Cover photograph (Copyright © 2007, American Society for Microbiology. All Rights Reserved.): High-throughput single cell analysis by multispectral imaging flow cytometry reveals that intracellular Kaposi's sarcoma-associated herpesvirus viral load correlates with the loss of immune synapse components. Two days postinfection, monoclonal antibodies detect surface expression of MHC-I (blue) on HeLa cells, while nuclear DNA is stained red with DRAQ5. Each intranuclear dot (yellow) represents monoclonal antibody detection of a multimer of LANA proteins associated with a single intracellular copy of the viral genome. (See related articles on page 5079 and in October 2006: vol. 80, issue 20, page 10073.)