Cover photograph (Copyright © 2001, American Society for Microbiology. All rights reserved.): Microtubule reorganization during herpes simplex virus type 1 (HSV-1) infection facilitates the nuclear localization of VP22, a major virion tegument protein. African green monkey kidney cells synchronously infected with HSV-1 strain F show microtubule rearrangement (red), resulting in the loss of microtubule organizing centers with the concomitant nuclear localization of VP22 (green), by indirect immunofluorescence staining. After fixation of the infected cells with (methanol-free) formaldehyde and permeabilization with acetone, microtubules and VP22 were detected using a monoclonal antibody specific for -tubulin (DM A1) and an affinity-purified polyclonal antibody specific for the viral protein (RGST49), respectively. Selective regulation of the subcellular localization of major viral structural proteins by cytoplasmic structures may represent a novel mechanism for modulating infectious virion assembly. (See related article in September 2001: vol. 75, no. 18, p. 8697.)