PrP Expression Level and Sensitivity to Prion Infection
- B. W. Caughey, Editor
ABSTRACT
Mice overexpressing the prion protein (PrP) sequence from various host species are widely used for measuring infectious titers in prion disease. However, the impact that the transgene expression level might have on the susceptibility to infection raises some concerns about the final biological relevance of these models. Here we report that endpoint titration of a sheep scrapie isolate in sheep and in mice overexpressing the ovine PrP results in similar estimates of the infectious titer.
FOOTNOTES
- Received 17 February 2014.
- Accepted 21 February 2014.
- Address correspondence to Olivier Andreoletti, o.andreoletti{at}envt.fr.
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J.-Y. Douet and C. Lacroux contributed equally to this article.
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Published ahead of print 26 February 2014
- Copyright © 2014, American Society for Microbiology. All Rights Reserved.
