Amino Acid Exchanges in the Putative Nuclear Export Signal of Adenovirus Type 5 L4-100K Severely Reduce Viral Progeny due to Effects on Hexon Biogenesis
ABSTRACT
The adenovirus type 5 nonstructural L4-100K protein is indispensable for efficient lytic infection. During the late phase, L4-100K promotes selective translation of viral late transcripts and mediates the trimerization of the major capsid protein hexon. In the present study, the role of a potential nuclear export signal in L4-100K was investigated. Intriguingly, amino acid substitutions in this sequence resulted in severely diminished progeny virus production, seemingly by precluding proper hexon biogenesis.
FOOTNOTES
- Received 7 August 2012.
- Accepted 8 November 2012.
- Address correspondence to Thomas Dobner, thomas.dobner{at}hpi.uni-hamburg.de.
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↵* Present address: Orkide O. Koyuncu, Princeton University, Department of Molecular Biology, Princeton, New Jersey, USA; Melanie Schmid, European Molecular Biology Laboratory, Heidelberg, Germany.
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Published ahead of print 21 November 2012
- Copyright © 2013, American Society for Microbiology. All Rights Reserved.
