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Journal of Virology, October 2005, p. 12602-12607, Vol. 79, No. 19
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.19.12602-12607.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

The Bunyamwera Virus mRNA Transcription Signal Resides within both the 3' and the 5' Terminal Regions and Allows Ambisense Transcription from a Model RNA Segment

John N. Barr,* John W. Rodgers, and Gail W. Wertz

Department of Microbiology, University of Alabama School of Medicine, Birmingham, Alabama

Received 11 May 2005/ Accepted 15 July 2005

Bunyamwera virus (BUNV) is the prototype of the Bunyaviridae family of RNA viruses. BUNV genomic strands are templates for both replication and transcription, whereas the antigenomic RNAs serve only as templates for replication. By mutagenesis of model templates, we showed that the BUNV transcription promoter comprises elements within both the 3' and the 5' nontranslated regions. Using this information, we designed a model ambisense BUNV segment that transcribed BUNV S mRNA from the genomic strand and green fluorescent protein (GFP) mRNA from the antigenome. Demonstration of GFP expression showed that this ambisense strategy represents a viable approach for generating BUNV segments able to express additional proteins.

* Corresponding author. Mailing address: Department of Microbiology, University of Alabama School of Medicine, BBRB Room 360/Box 17, 845 19th Street South, Birmingham AL 35294-2170. Phone: (205) 934-0453. Fax: (205) 934-1636. E-mail: jbarr{at}uab.edu.

Journal of Virology, October 2005, p. 12602-12607, Vol. 79, No. 19
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.19.12602-12607.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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