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J. Virol. doi:10.1128/JVI.02657-07
Copyright (c) 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Human CAR Gene Expression in Non-permissive Hamster Cells Boosts Entry of Type 12 Adenovirions and Nuclear Import of Viral DNA

Institute for Virology, Erlangen University Medical School, Schlossgarten 4, D-91054 Erlangen, Germany; Institute of Genetics, University of Cologne, Zülpicher Strasse 47, D-50674 Cologne, Germany; Friedrich Loeffler Institute for Medical Microbiology, University of Greifswald, Lutherstrasse 6, D-17487 Greifswald, Germany

^* To whom correspondence should be addressed. Email: walter.doerfler{at}viro.med.uni-erlangen.de.

	Abstract

Adenovirus 12 (Ad12) propagation in hamster BHK21 cells is blocked prior to viral DNA replication. The amounts of Ad12 DNA in the nuclei or cytoplasm of hamster cells are about 2 (2 h p.i.) and 4 to 5 orders of magnitude (48 h p.i.) lower than in permissive human cells. Cell line BHK21-hCAR is transgenic for and expresses the human Coxsackie and Adenovirus Receptor (CAR) gene. Nuclear uptake of Ad12 DNA in BHK21-hCAR cells is markedly increased compared to naïve BHK21 cells. Ad12 elicits a cytopathic effect in BHK21-hCAR, not in BHK21 cells. Q-PCR or ³H-thymidine labeling followed by zone velocity sedimentation fail to detect Ad12 DNA replication in BHK21 or BHK21-hCAR cells. Newly assembled Ad12 virions cannot be detected. Thus, the block in Ad12 DNA replication in hamster cells is not released by enhanced nuclear import of Ad12 DNA.