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J. Virol. doi:10.1128/JVI.02629-06
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

The Adenovirus E4 ORF3 Protein Binds and Reorganizes the TRIM Family Member Transcriptional Intermediary Factor 1 Alpha

Department of Molecular Genetics and Microbiology, School of Medicine, Stony Brook University, Stony Brook, New York 11794

^* To whom correspondence should be addressed. Email: phearing{at}ms.cc.sunysb.edu.

	Abstract

One of the most interesting functions attributed to the adenovirus early region 4 open reading frame 3 protein (E4 ORF3) is its reorganization of PML nuclear bodies. These normally punctate structures are reorganized by E4 ORF3 into tracks that eventually surround viral replication centers. PML rearrangement is an evolutionarily conserved function of E4 ORF3, yet its cause and functional relevance remain a mystery. The E4 ORF3 protein coimmunoprecipitates with the PML protein, yet E4 ORF3 still forms tracks in cells that lack PML. The PML protein is a member of a larger protein family termed Tripartite Motif (TRIM) proteins. TRIM proteins contain a tripartite domain structure in proximity to their N-termini that consists of a RING-finger domain, followed by one or two B-box domains and a C-terminal Coiled-Coil domain (collectively termed the RBCC domain). The order and spacing of these domains is evolutionarily conserved and thought to mediate protein:protein interactions and other functions. We implemented a proteomic approach to isolate cellular proteins that bind to E4 ORF3. We identified a novel interaction between E4 ORF3 and another TRIM family member, Transcriptional Intermediary Factor 1 alpha (TIF1). TIF1 functions by recruiting coactivators and/or corepressors to modulate transcription. The interaction between E4 ORF3 and TIF1 was validated by coimmunoprecipitation and binding of recombinant proteins. Indirect immunofluoresence demonstrated that TIF1 is reorganized into track-like structures that contain PML upon E4 ORF3 expression. The RBCC domain of TIF1 is sufficient for E4 ORF3-induced rearrangement and TIF1 reorganization is conserved across adenovirus serotypes.

This article has been cited by other articles:

• Ullman, A. J., Hearing, P. (2008). Cellular Proteins PML and Daxx Mediate an Innate Antiviral Defense Antagonized by the Adenovirus E4 ORF3 Protein. J. Virol. 82: 7325-7335 [Abstract] [Full Text]