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J. Virol. doi:10.1128/JVI.02445-07
Copyright (c) 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

PBS-dependent Restriction of Murine Leukemia Virus Requires HP1 Binding by TRIM28

Department of Biochemistry and Molecular Biophysics, Howard Hughes Medical Institute, Columbia University, College of Physicians and Surgeons, New York, NY 10032 USA; Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/ULP/Collège de France, BP10142, 67404 Illkirch-Cedex, France

^* To whom correspondence should be addressed. Email: goff{at}cancercenter.columbia.edu.

	Abstract

TRIM28 is a transcriptional co-repressor which is required for primer bind site (PBS)-dependent restriction of murine leukemia virus (MLV) replication in embryonic stem (ES) and embryonic carcinoma (EC) cells. PBS-dependent restriction of MLV leads to transcriptional silencing of the integrated provirus and has been shown to correlate with TRIM28-mediated recruitment of HP1 to the silenced loci. Here we show using a cell line with a point mutation in the HP1 binding domain of TRIM28 that interaction with HP1 is absolutely required for PBS-dependent restriction of MLV in the F9 EC cell line.

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