JVI Accepts, published online ahead of print on 23 January 2008

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J. Virol. doi:10.1128/JVI.02395-07
Copyright (c) 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Herpes Simplex Virus ICP27 Increases Translation of a Subset of Viral Late mRNAs

Errin C. Fontaine-Rodriguez and David M. Knipe^*

Department of Microbiology and Molecular Genetics and Program in Virology, Harvard Medical School, Boston, MA 02115

^* To whom correspondence should be addressed. Email: david_knipe{at}hms.harvard.edu.

The herpes simplex virus (HSV) ICP27 immediate-early (IE) protein plays an essential role in the expression of viral late genes. ICP27 is a multifunctional protein and has been reported to regulate multiple steps of mRNA synthesis and processing, including transcription, splicing, and nuclear export. Recently, ICP27 was reported to interact with translation factors and to stimulate translation of the viral late mRNA encoding VP16. We examined the effects of ICP27 on accumulation, nuclear export, and translation of HSV-1 late mRNAs encoding VP16, ICP5, and gD. We confirm here that ICP27 stimulates translation of VP16, as well as an additional HSV-1 late protein ICP5. Data presented here demonstrate that translation levels of both VP16 and ICP5 mRNA is reduced during infections with the ICP27 null virus mutant d27-1, and with ICP27 C-terminal deletion mutant viruses n406 and n504, as compared to WT virus. In contrast, translation of gD mRNA is not affected by the presence of ICP27 during infection. These data demonstrate that ICP27 functions to increase the translation levels of a subset of HSV-1 late genes, and this function requires the C-terminus of ICP27.

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