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J. Virol. doi:10.1128/JVI.02307-06
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Severe Acute Respiratory Syndrome Coronavirus Accessory Protein 6 Is a Virion-Associated Protein and Is Released from 6 Protein-Expressing Cells

Departments of Microbiology and Immunology, and Pathology, The University of Texas Medical Branch at Galveston, Galveston, Texas 77555-1019

^* To whom correspondence should be addressed. Email: shmakino{at}utmb.edu.

	Abstract

Analysis of severe acute respiratory syndrome coronavirus (SCoV) by either sucrose gradient equilibrium centrifugation or a virus capture assay using an anti-SCoV S protein antibody demonstrated that SCoV 6 protein, which is one of SCoV's accessory proteins, was incorporated into virus particles. Co-expression of SCoV S, M, E and 6 proteins was sufficient for incorporation of the 6 protein into virus-like particles. Cells transfected with plasmid expressing the 6 protein released SCoV 6 protein; however infected cells released SCoV 6 protein only in association with SCoV particles.

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