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J. Virol. doi:10.1128/JVI.02187-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

THE C3-V4 REGION IS A MAJOR TARGET OF AUTOLOGOUS NEUTRALIZING ANTIBODIES IN HIV-1 SUBTYPE C INFECTION

AIDS Virus Research Unit, National Institute for Communicable Diseases, Johannesburg, South Africa. Centre for the AIDS Programme of Research in South Africa (CAPRISA), University of KwaZulu Natal, Durban South Africa. Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Cape Town, South Africa

^* To whom correspondence should be addressed. Email: lynnm{at}nicd.ac.za.

	Abstract

The early autologous neutralizing antibody response in HIV-1 subtype C infections is often characterized by high titers, but the response is type-specific with little to no cross-neutralizing activity. The specificities of these early neutralizing antibodies are not known, however the type specificity suggests they may target the variable regions of the envelope. Here we show that cross-reactive anti-V3 antibodies developed within 3-12 weeks in 6 individuals but did not mediate autologous neutralization. Using a series of chimeric viruses, we found that antibodies directed at the V1V2, V4 and V5 regions contributed to autologous neutralization in some individuals, with V1V2 playing a more substantial role. However, these antibodies did not account for the total neutralizing capacity of these sera against the early autologous virus. Antibodies directed against the C3-V4 region were involved in autologous neutralization in all 4 sera studied. In two sera, transfer of the C3-V4 region rendered the chimera as sensitive to antibody neutralization as the parental virus. Although the C3 region, which contains the highly variable alpha 2-helix was not a direct target in most cases, it contributed to the formation of neutralization epitopes as substitution of this region resulted in neutralization resistance. These data suggest that the C3 and V4 regions combine to form important structural motifs and that epitopes in this region are major targets of the early autologous neutralizing response in HIV-1 subtype C infection.

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