JVI Accepts, published online ahead of print on 12 November 2008
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fabry, C. M.S
Right arrow Articles by Schoehn, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fabry, C. M.S
Right arrow Articles by Schoehn, G.

 Previous Article  |  Next Article 

J. Virol. doi:10.1128/JVI.01808-08
Copyright (c) 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

The C-terminal domains of Adenovirus serotype 5 protein IX assemble into an anti-parallel structure on the facets of the capsid

Céline M.S Fabry, Manuel Rosa-Calatrava, Christine Moriscot, Rob W.H. Ruigrok, Pierre Boulanger, and Guy Schoehn*

Université Joseph Fourier, Unit for Virus Host Cell Interaction, UMR 5233 UJF-EMBL-CNRS, BP 181, 38042 Grenoble cedex 9, France; Université Lyon, Faculté de Médecine Laennec, Laboratoire de Virologie et Pathologie Humaine, CNRS-FRE-3011 VirPath, 7, Rue Guillaume Paradin, F-69372 Lyon Cedex 08, France; Institut de Biologie Structurale Jean-Pierre Ebel, UMR5075 CEA-CNRS-Université Joseph Fourier, 41 rue Jules Horowitz, 38027 Grenoble cedex 1, France; Laboratoire de Virologie Médicale, Centre de Biologie et Pathologie Est, Hospices Civils de Lyon, 59, Boulevard Pinel, F-69677 Bron, France

* To whom correspondence should be addressed. Email: schoehn{at}embl.fr.


arrow
Abstract

Adenovirus serotype 5 protein IX (pIX) has two domains connected by a flexible linker. Three N-terminal domains form triskelions on the capsid facets that cement hexons together and the C-terminal domains of four monomers form complexes towards the facet periphery. Here we present a cryo-EM structure of recombinant adenovirus with a peptide tag added to the C-terminus of pIX. The structure made up by several C-termini of pIX is longer at both ends compared to wt protein and Fabs directed against the tag bind to both ends of the oligomer, demonstrating that the pIX C-termini associate in an anti-parallel manner.




This article has been cited by other articles:

  • Silvestry, M., Lindert, S., Smith, J. G., Maier, O., Wiethoff, C. M., Nemerow, G. R., Stewart, P. L. (2009). Cryo-Electron Microscopy Structure of Adenovirus Type 2 Temperature-Sensitive Mutant 1 Reveals Insight into the Cell Entry Defect. J. Virol. 83: 7375-7383 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»