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J. Virol. doi:10.1128/JVI.01780-06
Copyright (c) 2006, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Not all cytokine-producing CD8+ T-cells suppress simian immunodeficiency virus replication

Wisconsin National Primate Research Center; and Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, Wisconsin 53715-1299, Department of Biostatistics, University of Alabama at Birmingham, Birmingham, AL 35294

^* To whom correspondence should be addressed. Email: watkins{at}primate.wisc.edu.

	Abstract

Current assays of CD8+ T-lymphocyte function measure cytokine production rather than the ability of these lymphocytes to suppress viral replication. Here, we show that CD8+ T-cell clones recognizing the same epitope vary enormously in their ability to suppress SIVmac239 replication in an in vitro suppression assay. However, all Nef_165-173IW9- and Vif_66-73HW8-specific clones from elite controllers effectively suppressed SIV replication. Interestingly, in vitro suppression efficacy was not always associated with the ability to produce IFN-, TNF- or IL-2.

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