JVI Accepts, published online ahead of print on 10 October 2007

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J. Virol. doi:10.1128/JVI.01745-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Addition of deoxynucleosides enhances HIV-1 integration and 2LTR formation in resting CD4+ T cells

Gabriela Plesa, Jihong Dai, Cliff Baytop, James L. Riley, Carl H. June, and Una O'Doherty^*

Department of Pathology and Laboratory Medicine, Division of Transfusion Medicine, University of Pennsylvania, Philadelphia, PA 19104

^* To whom correspondence should be addressed. Email: unao{at}mail.med.upenn.edu.

Resting CD4+ T cells restrict HIV infection at a step prior to integration. Despite this restriction, we showed previously that HIV integration occurs in resting CD4+ T cells in vitro, albeit at lower levels than in activated CD4+ T cells. Here we show that addition of deoxynucleosides enhanced integration and 2LTR formation in resting CD4+ T cells, but the kinetics were still significantly delayed compared to activated T cells. Thus, deoxynucleoside addition partially overcomes restriction to HIV infection in resting CD4+ T cells.

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