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J. Virol. doi:10.1128/JVI.01663-06
Copyright (c) 2006, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Infectious RNA Isolated from the Spinal Cords of Mice Chronically Infected with Theiler's Murine Encephalomyelitis Virus

Department of Neurology, University of Utah School of Medicine, 30 North 1900 East, 3R330 SOM, Salt Lake City, UT 84132-2305; Department of Neuropharmacology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037

^* To whom correspondence should be addressed. Email: Robert.Fujinami{at}hsc.utah.edu.

	Abstract

The DA strain of Theiler's murine encephalomyelitis virus (TMEV) persistently infects cells of the spinal cord during the chronic phase of infection. Though in situ hybridization and RT-PCR have demonstrated the presence of viral RNA in the spinal cord, it has not been determined whether this RNA is infectious and, if so, how many PFU equivalents of virus the RNA can yield. In this study, we demonstrated that TMEV RNA isolated from the spinal cord of chronically infected mice is infectious and there is at least 30-fold more infectious RNA in the spinal cords of these mice than infectious virus.