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J. Virol. doi:10.1128/JVI.01399-06
Copyright (c) 2006, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Non-Neutralizing HRV2-Specific Monoclonal Antibody 2G2 Attaches to a Region that Undergoes Most Dramatic Changes Upon Release of the Viral RNA

Institut de Biologie Structurale Jean-Pierre Ebel UMR 5057 CEA-CNRS-UJF, 41 rue Jules Horowitz, 38027 Grenoble, France; Max F. Perutz Laboratories, Vienna Biocenter, Medical University of Vienna, Austria

^* To whom correspondence should be addressed. Email: hewat{at}ibs.fr,

	Abstract

The monoclonal antibody 2G2 has been extensively used for detection and quantification of structural changes of human rhinovirus serotype 2 during infection. It recognizes exclusively A and B subviral particles but not native virus. We have elucidated the basis of this selectivity by determining the footprint of 2G2. Since viral escape mutants can obviously not be obtained, the structure of complexes between Fab fragments of 2G2 and 80S subviral B particles were determined by cryo-electron microscopy. The footprint of the antibody corresponds to the capsid region that we predicted to undergo the most dramatic changes upon RNA release.