JVI Accepts, published online ahead of print on 6 August 2008
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J. Virol. doi:10.1128/JVI.01204-08
Copyright (c) 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Fusion of EGFP to the Pseudorabies Virus Axonal Sorting Protein, Us9, Blocks Anterograde Spread of Infection in Mammalian Neurons

M. G. Lyman, D. Curanovic, A. D. Brideau, and L. W. Enquist*

Department of Molecular Biology, Princeton University, Princeton NJ 08544

* To whom correspondence should be addressed. Email: lenquist{at}princeton.edu.


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Abstract

Pseudorabies virus encodes a membrane protein (Us9) that is essential for the axonal sorting of virus particles within neurons, and anterograde spread in the mammalian nervous system. GFP-tagged Us9 mimicked the trafficking properties of the wild-type protein in non-neuronal cells. We constructed a PRV strain that expressed Us9-EGFP, and tested its spread capabilities in the rat visual system and in primary neuronal cultures. We report that Us9-EGFP does not promote anterograde spread of infection, and may disrupt packing of viral membrane proteins in lipid rafts, an essential step for Us9-mediated axonal sorting.




This article has been cited by other articles:

  • McGraw, H. M., Awasthi, S., Wojcechowskyj, J. A., Friedman, H. M. (2009). Anterograde Spread of Herpes Simplex Virus Type 1 Requires Glycoprotein E and Glycoprotein I but Not Us9. J. Virol. 83: 8315-8326 [Abstract] [Full Text]  
  • Lyman, M. G., Kemp, C. D., Taylor, M. P., Enquist, L. W. (2009). Comparison of the Pseudorabies Virus Us9 Protein with Homologs from Other Veterinary and Human Alphaherpesviruses. J. Virol. 83: 6978-6986 [Abstract] [Full Text]  


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