JVI Accepts, published online ahead of print on 22 August 2007

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J. Virol. doi:10.1128/JVI.01133-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Herpes Simplex Virus Mutants with Multiple Substitutions Affecting DNA binding of UL42 Are Impaired for Viral Replication and DNA Synthesis

Changying Jiang, Ying T. Hwang, Guangliang Wang, John C. W. Randell, Donald M. Coen, and Charles B. C. Hwang^*

Department of Microbiology and Immunology, State University of New York Upstate Medical University, Syracuse, NY 13210, and Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115

^* To whom correspondence should be addressed. Email: hwangc{at}upstate.edu.

Herpes simplex virus mutants with single substitutions that decrease DNA binding by the DNA polymerase processivity subunit UL42 are only modestly impaired for viral replication. In this study, recombinant viruses harboring two or four of these mutations were constructed. The more substitutions, the more severe the defects in viral replication and DNA synthesis, suggesting that DNA binding by UL42 is important for these processes.

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