JVI Accepts, published online ahead of print on 25 July 2007

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Xu, N. Articles by Akusjärvi, G. Search for Related Content PubMed PubMed Citation Articles by Xu, N. Articles by Akusjärvi, G.

 Previous Article  |  Next Article 

J. Virol. doi:10.1128/JVI.00885-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Adenovirus VA RNAII derived small RNAs are efficiently incorporated into RISC and associate with polyribosomes

Ning Xu, Bo Segerman, Xiaofu Zhou, and Göran Akusjärvi^*

Department of Medical Biochemistry and Microbiology, Uppsala Biomedical Center, Husargatan 3, S-751 23 Uppsala, Sweden

^* To whom correspondence should be addressed. Email: goran.akusjarvi{at}imbim.uu.se.

Adenovirus type 5 encodes two highly structured short RNAs, the virus-associated RNAs, VA RNAI and VA RNAII. Both are processed by Dicer into small RNAs that are incorporated into the RNA-induced silencing complex (RISC). We here show, by cloning of small RNAs, that approximately 80% of Ago2-containing RISC immunopurified from late infected cells, is associated with VA RNA-derived small RNAs (mivaRNAs). Most surprisingly, VA RNAII, which is expressed at 20-fold lower levels compared to VA RNAI, appears to be the preferred substrate for Dicer and accounts for approximately 60% of all small RNAs in RISC. The mivaRNAs are derived from the 3' strand of the terminal stems of the VA RNAs, with the major fraction of VA RNAII starting at position 138. The small RNAs derived from VA RNAI were more heterogeneous in size with the two predominant small RNAs starting at position 137 and 138. Collectively, our results suggest that the mivaRNAs are efficiently used for RISC assembly in late infected cells. Potentially they function as miRNAs regulating translation of cellular mRNAs. In support of this hypothesis, we detect a fraction of the VA RNAII derived mivaRNAs on polyribosomes

This article has been cited by other articles:

• Aparicio, O., Carnero, E., Abad, X., Razquin, N., Guruceaga, E., Segura, V., Fortes, P. (2009). Adenovirus VA RNA-derived miRNAs target cellular genes involved in cell growth, gene expression and DNA repair. Nucleic Acids Res 0: gkp1028v1-gkp1028 [Abstract] [Full Text]  
• Xu, N., Gkountela, S., Saeed, K., Akusjarvi, G. (2009). The 5'-end heterogeneity of adenovirus virus-associated RNAI contributes to the asymmetric guide strand incorporation into the RNA-induced silencing complex. Nucleic Acids Res 37: 6950-6959 [Abstract] [Full Text]  
• Iwakawa, H.-o., Mizumoto, H., Nagano, H., Imoto, Y., Takigawa, K., Sarawaneeyaruk, S., Kaido, M., Mise, K., Okuno, T. (2008). A Viral Noncoding RNA Generated by cis-Element-Mediated Protection against 5'->3' RNA Decay Represses both Cap-Independent and Cap-Dependent Translation. J. Virol. 82: 10162-10174 [Abstract] [Full Text]