JVI Accepts, published online ahead of print on 24 June 2009

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J. Virol. doi:10.1128/JVI.00786-09
Copyright (c) 2009, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Computational analysis of human adenovirus type 22 provides evidence for recombination between human adenoviruses species D in the penton base gene

Christopher M. Robinson, Jaya Rajaiya, Michael P. Walsh, Donald Seto, David W. Dyer, Morris S. Jones, and James Chodosh^*

Department of Ophthalmology, Howe Laboratory, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, Massachusetts; Department of Bioinformatics and Computational Biology, George Mason University, Manassas, VA; Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma; Clinical Investigation Facility, David Grant USAF Medical Center, Travis, California

^* To whom correspondence should be addressed. Email: james_chodosh{at}meei.harvard.edu.

Recombination in human adenoviruses (HAdV) may confer virulence to an otherwise nonvirulent strain. The genome sequence of HAdV species D type 22 (HAdV-D22) revealed evidence for recombination with HAdV-D19 and HAdV-D37 within the capsid penton base gene. Bootscan analysis demonstrated that recombination sites within the penton base gene frame the coding sequences for the two external hypervariable loops in the protein. A similar pattern of recombination was evident within other HAdV-D types but not other HAdV species. Further study of recombination between HAdVs is needed to better predict possible recombination events between wild type viruses and adenoviral gene therapy vectors.