JVI Accepts, published online ahead of print on 16 April 2008
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J. Virol. doi:10.1128/JVI.00247-08
Copyright (c) 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Construction and characterization of a Human T-cell Lymphotropic Virus Type-3 infectious molecular clone

Sébastien Alain Chevalier, Nga Ling Ko, Sara Calattini, Adeline Mallet, Marie-Christine Prévost, Kylene Kehn, John N. Brady, Fatah Kashanchi, Antoine Gessain, and Renaud Mahieux*

Unité d'Epidémiologie et Physiopathologie des Virus Oncogènes, CNRS URA 3015, Département de Virologie, Institut Pasteur, 28 rue du Dr Roux, 75015 Paris, France; Plateforme de Microscopie Electronique, Institut Pasteur, 25 rue du Dr Roux, 75015 Paris, France; Department of Microbiology, Immunology and Tropical Medicine and Department of Biochemistry, The George Washington University Medical Center, Washington, DC 20037, USA; NIH/NCI/LCO/VTB, Bethesda. MD, 20892, USA

* To whom correspondence should be addressed. Email: rmahieux{at}pasteur.fr.


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Abstract

We and others have uncovered the existence of HTLV-3. We have now generated an HTLV-3 proviral clone. We established that gag, env, pol, pro tax/rex as well as a minus strand mRNAs are present in cells transfected with the HTLV-3 clone. HTLV-3 p24gag protein is detected in the cell culture supernatant. Transfection of 293T-LTR-GFP cells with the HTLV-3 clone promotes syncytia formation, a hallmark of Env expression, together with the appearance of fluorescent cells, demonstrating that Tax is expressed. Viral particles are visible by electron microscopy. These particles are infectious, as demonstrated by infection experiments with purified virions.




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