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Journal of Virology, May 2009, p. 4678-4689, Vol. 83, No. 9
0022-538X/09/$08.00+0     doi:10.1128/JVI.02401-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Densovirus Infectious Pathway Requires Clathrin-Mediated Endocytosis Followed by Trafficking to the Nucleus {triangledown}

Agnès Vendeville,1,2 Marc Ravallec,2 Françoise-Xavière Jousset,2 Micheline Devise,1,2 Doriane Mutuel,2 Miguel López-Ferber,3 Philippe Fournier,2 Thierry Dupressoir,1,2 and Mylène Ogliastro2*

UMR 1231 BiVi, EPHE-Montpellier II,1 UMR 1231 INRA-Montpellier II, Montpellier,2 Ecole des Mines d'Alès, 30319 Alès, France3

Received 20 November 2008/ Accepted 10 February 2009

Junonia coenia densovirus (JcDNV) is an ambisense insect parvovirus highly pathogenic for lepidopteran pests at larval stages. The potential use of DNVs as biological control agents prompted us to reinvestigate the host range and cellular mechanisms of infection. In order to understand the early events of infection, we set up a functional infection assay in a cell line of the pest Lymantria dispar to determine the intracellular pathway undertaken by JcDNV to infect a permissive lepidopteran cell line. Our results show that JcDNV particles are rapidly internalized into clathrin-coated vesicles and slowly traffic within early and late endocytic compartments. Blocking late-endocytic trafficking or neutralizing the pH with drugs inhibited infection. During internalization, disruption of the cytoskeleton, and inhibition of phosphatidylinositol 3-kinase blocked the movement of vesicles containing the virus to the nucleus and impaired infection. In summary, our results define for the first time the early endocytic steps required for a productive DNV infection.

* Corresponding author. Mailing address: UMR 1231 INRA-Montpellier II, Place Eugène Bataillon, Bat. 24, cc101, 34095 Montpellier Cedex 5, France. Phone: 33 467 144 119. Fax: 33 467 144 299. E-mail: ogliastr{at}supagro.inra.fr

{triangledown} Published ahead of print on 18 February 2009.

Journal of Virology, May 2009, p. 4678-4689, Vol. 83, No. 9
0022-538X/09/$08.00+0     doi:10.1128/JVI.02401-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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