This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Laddy, D. J.
Right arrow Articles by Weiner, D. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Laddy, D. J.
Right arrow Articles by Weiner, D. B.

 Previous Article  |  Next Article 

Journal of Virology, May 2009, p. 4624-4630, Vol. 83, No. 9
0022-538X/09/$08.00+0     doi:10.1128/JVI.02335-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Electroporation of Synthetic DNA Antigens Offers Protection in Nonhuman Primates Challenged with Highly Pathogenic Avian Influenza Virus {triangledown}

Dominick J. Laddy,1 Jian Yan,1 Amir S. Khan,2 Hanne Andersen,3 Amanda Cohn,3 Jack Greenhouse,3 Mark Lewis,3 Jody Manischewitz,4 Lisa R. King,4 Hana Golding,4 Ruxandra Draghia-Akli,2 and David B. Weiner1*

Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104,1 VGX Pharmaceuticals, Inc., The Woodlands, Texas 77381,2 Bioqual, Inc., Rockville, Maryland 20850,3 U.S. Food and Drug Administration, Center for Biologics Evaluation and Research, Office of Vaccines Research and Review, Division of Viral Products, Bethesda, Maryland 208924

Received 8 November 2008/ Accepted 3 February 2009

Avian influenza highlights the need for novel vaccination techniques that would allow for the rapid design and production of safe and effective vaccines. An ideal platform would be capable of inducing both protective antibodies and potent cellular immune responses. These potential advantages of DNA vaccines remain unrealized due to a lack of efficacy in large animal studies and in human trials. Questions remain regarding the potential utility of cellular immune responses against influenza virus in primates. In this study, by construct optimization and in vivo electroporation of synthetic DNA-encoded antigens, we observed the induction of cross-reactive cellular and humoral immune responses individually capable of providing protection from influenza virus infection in the rhesus macaque. These studies advance the DNA vaccine field and provide a novel, more tolerable vaccine with broad immunogenicity to avian influenza virus. This approach appears important for further investigation, including studies with humans.

* Corresponding author. Mailing address: Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104. Phone: (215) 349-8591. Fax: (215) 573-9436. E-mail: dbweiner{at}mail.med.upenn.edu

{triangledown} Published ahead of print on 11 February 2009.

Journal of Virology, May 2009, p. 4624-4630, Vol. 83, No. 9
0022-538X/09/$08.00+0     doi:10.1128/JVI.02335-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»