This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kutluay, S. B.
Right arrow Articles by Triezenberg, S. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kutluay, S. B.
Right arrow Articles by Triezenberg, S. J.

 Previous Article  |  Next Article 

Journal of Virology, April 2009, p. 3436-3449, Vol. 83, No. 8
0022-538X/09/$08.00+0     doi:10.1128/JVI.02349-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Transcriptional Coactivators Are Not Required for Herpes Simplex Virus Type 1 Immediate-Early Gene Expression In Vitro{triangledown}

Sebla B. Kutluay,1,2 Sarah L. DeVos,2,{dagger} Jennifer E. Klomp,2 and Steven J. Triezenberg1,2,3*

Graduate Program in Cell and Molecular Biology,1 Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan 48824,3 Van Andel Research Institute, Grand Rapids, Michigan 495032

Received 11 November 2008/ Accepted 21 January 2009

Virion protein 16 (VP16) of herpes simplex virus type 1 (HSV-1) is a potent transcriptional activator of viral immediate-early (IE) genes. The VP16 activation domain can recruit various transcriptional coactivators to target gene promoters. However, the role of transcriptional coactivators in HSV-1 IE gene expression during lytic infection had not been fully defined. We showed previously that transcriptional coactivators such as the p300 and CBP histone acetyltransferases and the BRM and Brg-1 chromatin remodeling complexes are recruited to viral IE gene promoters in a manner dependent mostly on the presence of the activation domain of VP16. In this study, we tested the hypothesis that these transcriptional coactivators are required for viral IE gene expression during infection of cultured cells. The disrupted expression of the histone acetyltransferases p300, CBP, PCAF, and GCN5 or the BRM and Brg-1 chromatin remodeling complexes did not diminish IE gene expression. Furthermore, IE gene expression was not impaired in cell lines that lack functional p300, or BRM and Brg-1. We also tested whether these coactivators are required for the VP16-dependent induction of IE gene expression from transcriptionally inactive viral genomes associated with high levels of histones in cultured cells. We found that the disruption of coactivators also did not affect IE gene expression in this context. Thus, we conclude that the transcriptional coactivators that can be recruited by VP16 do not contribute significantly to IE gene expression during lytic infection or the induction of IE gene expression from nucleosomal templates in vitro.

* Corresponding author. Mailing address: Van Andel Research Institute, 333 Bostwick NE, Grand Rapids, MI 49503. Phone: (616) 234-5704. Fax: (616) 234-5709. E-mail: steve.triezenberg{at}vai.org

{triangledown} Published ahead of print on 28 January 2009.

{dagger} Present address: Central Michigan University, Mt. Pleasant, MI 48859.

Journal of Virology, April 2009, p. 3436-3449, Vol. 83, No. 8
0022-538X/09/$08.00+0     doi:10.1128/JVI.02349-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kutluay, S. B., Triezenberg, S. J. (2009). Regulation of Histone Deposition on the Herpes Simplex Virus Type 1 Genome during Lytic Infection. J. Virol. 83: 5835-5845 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»