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Journal of Virology, March 2009, p. 2274-2284, Vol. 83, No. 5
0022-538X/09/$08.00+0     doi:10.1128/JVI.01791-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Tax1BP1 Interacts with Papillomavirus E2 and Regulates E2-Dependent Transcription and Stability{triangledown}

Xiaoyu Wang, Samisubbu R. Naidu, Francis Sverdrup,{dagger} and Elliot J. Androphy*

Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605

Received 26 August 2008/ Accepted 12 December 2008

The papillomavirus E2 proteins regulate viral replication, gene transcription, and genome maintenance by interacting with other viral and host proteins. From a yeast two-hybrid screen, we identified the cellular protein Tax1BP1 as a novel binding partner of human papillomavirus type 18 (HPV18) E2. Tax1BP1 also interacts with the HPV16 and bovine papillomavirus type 1 (BPV1) E2 proteins, with the C-terminal region of Tax1BP1 interacting with the N-terminal transactivation domain of BPV1 E2. Tax1BP1 complexes with p300 and acts synergistically as a coactivator with p300 to enhance E2-dependent transcription. Using chromatin immunoprecipitation assays, we show that Tax1BP1 and E2 localize to the long control region on the BPV1 genome. Tax1BP1 was recently reported to bind ubiquitin and to function as an essential component of an A20 ubiquitin-editing complex. We demonstrate that Tax1BP1 plays a role in the regulation of the steady-state level of E2 by preventing its proteasomal degradation. These studies provide new insights into the regulation of E2 functions.

* Corresponding author. Mailing address: UMass Medical School/LRB 328, 364 Plantation St., Worcester, MA 01605. Phone: (508) 856-6605. Fax: (508) 856-6797. E-mail: elliot.androphy{at}umassmed.edu

{triangledown} Published ahead of print on 24 December 2008.

{dagger} Present address: Pfizer St. Louis, Chesterfield, MO 63017.

Journal of Virology, March 2009, p. 2274-2284, Vol. 83, No. 5
0022-538X/09/$08.00+0     doi:10.1128/JVI.01791-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Zheng, G., Schweiger, M.-R., Martinez-Noel, G., Zheng, L., Smith, J. A., Harper, J. W., Howley, P. M. (2009). Brd4 Regulation of Papillomavirus Protein E2 Stability. J. Virol. 83: 8683-8692 [Abstract] [Full Text]  


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