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Journal of Virology, February 2009, p. 1708-1717, Vol. 83, No. 4
0022-538X/09/$08.00+0     doi:10.1128/JVI.01364-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Vaccinia Virus Protein F12 Associates with Intracellular Enveloped Virions through an Interaction with A36{triangledown}

Sara C. Johnston and Brian M. Ward*

Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester, New York 14642

Received 30 June 2008/ Accepted 20 November 2008

Vaccinia virus is the prototypical member of the family Poxviridae. Three morphologically distinct forms are produced during infection: intracellular mature virions (IMV), intracellular enveloped virions (IEV), and extracellular enveloped virions (EEV). Two viral proteins, F12 and A36, are found exclusively on IEV but not on IMV and EEV. Analysis of membranes from infected cells showed that F12 was only associated with membranes and is not an integral membrane protein. A yeast two-hybrid assay revealed an interaction between amino acids 351 to 458 of F12 and amino acids 91 to 111 of A36. We generated a recombinant vaccinia virus that expresses an F12, which lacks residues 351 to 458. Characterization of this recombinant revealed a small-plaque phenotype and a subsequent defect in virus release similar to a recombinant virus that had F12L deleted. In addition, F12 lacking residues 351 to 458 was unable to associate with membranes in infected cells. These results suggest that F12 associates with IEV through an interaction with A36 and that this interaction is critical for the function of F12 during viral egress.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, University of Rochester Medical Center, 601 Elmwood Ave., Box 672, Rochester, NY 14642. Phone: (585) 275-9715. Fax: (585) 473-9573. E-mail: brian_ward{at}urmc.rochester.edu

{triangledown} Published ahead of print on 3 December 2008.

Journal of Virology, February 2009, p. 1708-1717, Vol. 83, No. 4
0022-538X/09/$08.00+0     doi:10.1128/JVI.01364-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Van Vliet, K., Mohamed, M. R., Zhang, L., Villa, N. Y., Werden, S. J., Liu, J., McFadden, G. (2009). Poxvirus Proteomics and Virus-Host Protein Interactions. Microbiol. Mol. Biol. Rev. 73: 730-749 [Abstract] [Full Text]  


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