This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Johansson, P.
Right arrow Articles by Rymo, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Johansson, P.
Right arrow Articles by Rymo, L.

 Previous Article  |  Next Article 

Journal of Virology, February 2009, p. 1393-1401, Vol. 83, No. 3
0022-538X/09/$08.00+0     doi:10.1128/JVI.01637-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Nuclear Factor-{kappa}B Binds to the Epstein-Barr Virus LMP1 Promoter and Upregulates Its Expression{triangledown}

Pegah Johansson, Ann Jansson, Ulla Rüetschi, and Lars Rymo*

Department of Clinical Chemistry and Transfusion Medicine, Sahlgrenska Academy at the University of Gothenburg, 413 45 Gothenburg, Sweden

Received 31 July 2008/ Accepted 7 November 2008

The latent membrane protein 1 (LMP1) oncogene carried by Epstein-Barr virus (EBV) is essential for transformation and maintenance of EBV-immortalized B cells in vitro, and it is expressed in most EBV-associated tumor types. The activation of the NF-{kappa}B pathway by LMP1 plays a critical role in the upregulation of antiapoptotic proteins. The EBV-encoded EBNA2 transactivator is required for LMP1 activation in latency III, while LMP1 itself appears to be critical for its activation in the latency II gene expression program. In both cases, additional viral and cellular transcription factors are required in mediating transcription activation of the LMP1 promoter. Using DNA affinity purification and chromatin immunoprecipitation assay, we showed here that members of the NF-{kappa}B transcription factor family bound to the LMP1 promoter in vitro and in vivo. Electrophoretic mobility shift assay analyses indicated the binding of the p50-p50 homodimer and the p65-p50 heterodimer to an NF-{kappa}B site in the LMP1 promoter. Transient transfections and reporter assays showed that the LMP1 promoter is activated by exogenous expression of NF-{kappa}B factors in both B cells and epithelial cells. Exogenous expression of NF-{kappa}B factors in the EBNA2-deficient P3HR1 cell line induced LMP1 protein expression. Overall, our data are consistent with the presence of a positive regulatory circuit between NF-{kappa}B activation and LMP1 expression.

* Corresponding author. Mailing address: Department of Clinical Chemistry and Transfusion Medicine, Sahlgrenska University Hospital, 413 45 Gothenburg, Sweden. Phone: 46 (0)31 3424080. Fax: 46 (0)31 828458. E-mail: lars.rymo{at}clinchem.gu.se

{triangledown} Published ahead of print on 19 November 2008.

Journal of Virology, February 2009, p. 1393-1401, Vol. 83, No. 3
0022-538X/09/$08.00+0     doi:10.1128/JVI.01637-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Demetriades, C., Mosialos, G. (2009). The LMP1 Promoter Can Be Transactivated Directly by NF-{kappa}B. J. Virol. 83: 5269-5277 [Abstract] [Full Text]  
  • Izumiya, Y., Izumiya, C., Hsia, D., Ellison, T. J., Luciw, P. A., Kung, H.-J. (2009). NF-{kappa}B Serves as a Cellular Sensor of Kaposi's Sarcoma-Associated Herpesvirus Latency and Negatively Regulates K-Rta by Antagonizing the RBP-J{kappa} Coactivator. J. Virol. 83: 4435-4446 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»