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Journal of Virology, February 2009, p. 1184-1192, Vol. 83, No. 3
0022-538X/09/$08.00+0     doi:10.1128/JVI.02010-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Efficient Nuclear Export of Herpes Simplex Virus 1 Transcripts Requires both RNA Binding by ICP27 and ICP27 Interaction with TAP/NXF1{triangledown}

Lisa A. Johnson and Rozanne M. Sandri-Goldin*

Department of Microbiology and Molecular Genetics, School of Medicine, University of California, Irvine, Irvine, California 92697-4025

Received 23 September 2008/ Accepted 13 November 2008

Herpes simplex virus 1 (HSV-1) regulatory protein ICP27 has been reported to bind viral RNA and to interact with the nuclear export adaptor Aly/REF and the major cellular mRNA nuclear export receptor TAP/NXF1. Using in situ hybridization and in vitro export assays, we show here that poly(A)+ RNA was retained in the nucleus of cells infected with viral ICP27 mutants that either cannot bind RNA or that do not interact with TAP/NXF1. Microarray analysis of nuclear and cytoplasmic RNA fractions demonstrated that efficient export of the majority of viral transcripts requires that ICP27 be able to bind RNA and to interact with TAP/NXF1. We conclude that ICP27 is the major export adaptor for HSV-1 mRNA and that it links bound transcripts to the TAP/NXF1 export receptor.

* Corresponding author. Mailing address: Department of Microbiology and Molecular Genetics, School of Medicine, Medical Sciences, B240, University of California, Irvine, CA 92697-4025. Phone: (949) 824-7570. Fax: (949) 824-9054. E-mail: rmsandri{at}uci.edu

{triangledown} Published ahead of print on 19 November 2008.

Journal of Virology, February 2009, p. 1184-1192, Vol. 83, No. 3
0022-538X/09/$08.00+0     doi:10.1128/JVI.02010-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Corbin-Lickfett, K. A., Chen, I-H. B., Cocco, M. J., Sandri-Goldin, R. M. (2009). The HSV-1 ICP27 RGG box specifically binds flexible, GC-rich sequences but not G-quartet structures. Nucleic Acids Res 0: gkp793v1-gkp793 [Abstract] [Full Text]  
  • Johnson, L. A., Li, L., Sandri-Goldin, R. M. (2009). The Cellular RNA Export Receptor TAP/NXF1 Is Required for ICP27-Mediated Export of Herpes Simplex Virus 1 RNA, but the TREX Complex Adaptor Protein Aly/REF Appears To Be Dispensable. J. Virol. 83: 6335-6346 [Abstract] [Full Text]  
  • Souki, S. K., Gershon, P. D., Sandri-Goldin, R. M. (2009). Arginine Methylation of the ICP27 RGG Box Regulates ICP27 Export and Is Required for Efficient Herpes Simplex Virus 1 Replication. J. Virol. 83: 5309-5320 [Abstract] [Full Text]  


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