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Journal of Virology, July 2009, p. 7353-7356, Vol. 83, No. 14
0022-538X/09/$08.00+0     doi:10.1128/JVI.00546-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Multiple Integrated Copies and High-Level Production of the Human Retrovirus XMRV (Xenotropic Murine Leukemia Virus-Related Virus) from 22Rv1 Prostate Carcinoma Cells{triangledown}

Emily C. Knouf, Michael J. Metzger, Patrick S. Mitchell, Jason D. Arroyo, John R. Chevillet, Muneesh Tewari, and A. Dusty Miller*

Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, Seattle, Washington 98109-1024

Received 17 March 2009/ Accepted 23 April 2009

The human retrovirus XMRV (xenotropic murine leukemia virus-related virus) is associated with prostate cancer, most frequently in humans with a defect in the antiviral defense protein RNase L, suggesting a role for XMRV in prostate carcinogenesis. However, XMRV has not been found in prostate carcinoma cells. Here we show that 22Rv1 prostate carcinoma cells produce high-titer virus that is nearly identical in properties and sequence to XMRV isolated by others and consist primarily of a single clone of cells with at least 10 integrated copies of XMRV, warranting further study of a possible role for XMRV integration in carcinogenesis.

* Corresponding author. Mailing address: Fred Hutchinson Cancer Research Center, 1100 Fairview Ave. N., Room C2-105, Seattle, WA 98109-1024. Phone: (206) 667-2890. Fax: (206) 667-6523. E-mail: dmiller{at}fhcrc.org

{triangledown} Published ahead of print on 29 April 2009.

Journal of Virology, July 2009, p. 7353-7356, Vol. 83, No. 14
0022-538X/09/$08.00+0     doi:10.1128/JVI.00546-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



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